Agrobacterium tumefaciens mediated genetic transformation and regeneration of Morus alba L.

被引：4
|
作者：
Agarwal, S
Kanwar, K [1 ]
Saini, N
Jain, RK
机构：
[1] Univ Hort & Forestry, Dept Biotechnol, Solan 173230, HP, India
[2] Haryana Agr Univ, Dept Biotechnol & Mol Biol, Hisar, India
来源：
SCIENTIA HORTICULTURAE | 2004年 / 100卷 / 1-4期
关键词：
genetic transformation; Morus alba L; shoot regeneration; tissue culture;
D O I：
10.1016/j.scienta.2003.06.002
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
An efficient method was established for genetic transformation of Morus alba clone M5 using Agrobacterium tumefaciens mediated gene transfer. Cotyledon explants from in vitro grown seedlings were co-cultivated with disarmed strain LBA 4404 harbouring the binary vector p(BI121) carrying chimeric beta-glucuronidase (GUS) and neomycin phosphotransferase (npt II) genes. Maximum transformation frequency of 18.60% was recorded with 48 It of pre-conditioning followed by co-cultivation for the same duration. Expression and presence of transgene was confirmed by histochemical test and polymerase chain reaction. The transgenic plants were micropropagated and successfully acclimatised. (C) 2003 Published by Elsevier B.V.
引用
下载
收藏
页码：183 / 191
页数：9
相关论文
共 50 条
  • [1] EFFICIENT REGENERATION AND AGROBACTERIUM TUMEFACIENS MEDIATED GENETIC TRANSFORMATION OF POTATO (SOLANUM TUBEROSUM L.)
    Ahmed, Hussein Abdullah
    Barpete, Surendra
    Akdogan, Guray
    Aydin, Gulsum
    Sancak, Cengiz
    Ozcan, Sebahattin
    FRESENIUS ENVIRONMENTAL BULLETIN, 2018, 27 (05): : 3020 - 3027
  • [2] Agrobacterium tumefaciens-mediated genetic transformation of Digitalis purpurea L.
    Naivy Pérez-Alonso
    Borys Chong-Pérez
    Alina Capote
    Anabel Pérez
    Yovanny Izquierdo
    Geert Angenon
    Elio Jiménez
    Plant Biotechnology Reports, 2014, 8 : 387 - 397
  • [3] Agrobacterium tumefaciens-mediated genetic transformation of Digitalis purpurea L.
    Perez-Alonso, Naivy
    Chong-Perez, Borys
    Capote, Alina
    Perez, Anabel
    Izquierdo, Yovanny
    Angenon, Geert
    Jimenez, Elio
    PLANT BIOTECHNOLOGY REPORTS, 2014, 8 (05) : 387 - 397
  • [4] Genetic transformation of pear Pyrus communis L. mediated by Agrobacterium tumefaciens
    Merkulov, SM
    Bartish, IV
    Dolgov, SV
    Pasternak, TP
    McHugen, A
    GENETIKA, 1998, 34 (03): : 373 - 378
  • [5] Comparison of genetic transformation in Morus alba L. via different regeneration systems
    Sandhya Agarwal
    Kamlesh Kanwar
    Plant Cell Reports, 2007, 26 : 177 - 185
  • [6] Comparison of genetic transformation in Morus alba L. via different regeneration systems
    Agarwal, Sandhya
    Kanwar, Kamlesh
    PLANT CELL REPORTS, 2007, 26 (02) : 177 - 185
  • [7] A preliminary attempt for efficient genetic transformation and regeneration of legume Mucuna pruriens L. mediated by Agrobacterium tumefaciens
    Sathyanarayana, Raghavendra
    Kumar, Vadlapudi
    Ramesh, Chapeyil Kumaran
    Parmesha, Mahadevappa
    Khan, Mahaboob Habeebulla Moinuddin
    TURKISH JOURNAL OF BIOLOGY, 2012, 36 (03) : 285 - 292
  • [8] Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens
    Jonoubi, P
    Mousavi, A
    Majd, A
    Salmanian, AH
    Javaran, MJ
    Daneshian, J
    BIOLOGIA PLANTARUM, 2005, 49 (02) : 175 - 180
  • [9] Agrobacterium tumefaciens-mediated genetic transformation of rye (Secale cereale L.)
    Popelka, JC
    Altpeter, F
    MOLECULAR BREEDING, 2003, 11 (03) : 203 - 211
  • [10] Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)
    Yadav, Manju
    Chaudhary, Darshna
    Sainger, Manish
    Jaiwal, Pawan K.
    PLANT CELL TISSUE AND ORGAN CULTURE, 2010, 103 (03) : 377 - 386
12345