Areas of Application and Characteristics of Quantitative Chromatographic Analysis by the Consecutive Standard Addition Method

Quantitative chromatographic analysis by the standard addition method was recommended for determining analytes in the samples whose matrices possess sorption properties. It was found that the use of several consecutive additives increased the accuracy of results, and it is applicable under the conditions of the nonlinear dependence of detector signals on the analyte amount, in particular, in the HPLC analysis with electrospray ionization and mass-spectrometric detection. Different dependences of the analyte concentrations to be determined on the total amounts of additives were established; because of this, the results should be extrapolated to either zero or infinitely large amounts of additives. A criterion was proposed for predicting a method for the extrapolation of experimental data.