Persistent Wolbachia and Cultivable Bacteria Infection in the Reproductive and Somatic Tissues of the Mosquito Vector Aedes albopictus

被引:89
|
作者
Zouache, Karima
Voronin, Denis
Tran-Van, Van
Mousson, Laurence
Failloux, Anna-Bella
Mavingui, Patrick
机构
[1] Université de Lyon, Lyon
[2] Université Lyon 1 Villeurbanne, CNRS, UMR5557, Lyon
[3] Institut Pasteur Génétique Moléculaire des Bunyavirus, Paris
来源
PLOS ONE | 2009年 / 4卷 / 07期
关键词
INDUCED CYTOPLASMIC INCOMPATIBILITY; IN-SITU HYBRIDIZATION; ENDOSYMBIONT WOLBACHIA; INSECTICIDE RESISTANCE; ANOPHELES-GAMBIAE; MIDGUT BACTERIA; GENUS ASAIA; DIPTERA; POPULATIONS; CHIKUNGUNYA;
D O I
10.1371/journal.pone.0006388
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Commensal and symbiotic microbes have a considerable impact on the behavior of many arthropod hosts, including hematophagous species that transmit pathogens causing infectious diseases to human and animals. Little is known about the bacteria associated with mosquitoes other than the vectorized pathogens. This study investigated Wolbachia and cultivable bacteria that persist through generations in Ae. albopictus organs known to host transmitted arboviruses, such as dengue and chikungunya. Methodology/Principal Findings: We used culturing, diagnostic and quantitative PCR, as well as in situ hybridization, to detect and locate bacteria in whole individual mosquitoes and in dissected tissues. Wolbachia, cultivable bacteria of the genera Acinetobacter, Comamonas, Delftia and Pseudomonas co-occurred and persisted in the bodies of both males and females of Ae. albopictus initially collected in La Reunion during the chikungunya outbreak, and maintained as colonies in insectaries. In dissected tissues, Wolbachia and the cultivable Acinetobacter can be detected in the salivary glands. The other bacteria are commonly found in the gut. Quantitative PCR estimates suggest that Wolbachia densities are highest in ovaries, lower than those of Acinetobacter in the gut, and approximately equal to those of Acinetobacter in the salivary glands. Hybridization using specific fluorescent probes successfully localized Wolbachia in all germ cells, including the oocytes, and in the salivary glands, whereas the Acinetobacter hybridizing signal was mostly located in the foregut and in the anterior midgut. Conclusions/Significance: Our results show that Proteobacteria are distributed in the somatic and reproductive tissues of mosquito where transmissible pathogens reside and replicate. This location may portend the coexistence of symbionts and pathogens, and thus the possibility that competition or cooperation phenomena may occur in the mosquito vector Ae. albopictus. Improved understanding of the vectorial system, including the role of bacteria in the vector's biology and competence, could have major implications for understanding viral emergences and for disease control.
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页数:10
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