Production of single-cell protein with two-step fermentation for treatment of potato starch processing waste

被引:38
|
作者
Liu, Bingnan [1 ]
Li, Ying [1 ]
Song, Jinzhu [1 ]
Zhang, Lihong [1 ]
Dong, Jiacheng [1 ]
Yang, Qian [1 ,2 ]
机构
[1] Harbin Inst Technol, Sch Life Sci & Technol, Ctr Bioengn, Harbin 150001, Peoples R China
[2] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China
关键词
SOLID-STATE FERMENTATION; BACILLUS-LICHENIFORMIS; ASPERGILLUS-NIGER; BY-PRODUCTS; FOOD; BIODEGRADATION; BIOCONVERSION; TEMPERATURE; INDUSTRY; MILK;
D O I
10.1007/s10570-014-0400-6
中图分类号
TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];
学科分类号
0805 ; 080502 ; 0822 ;
摘要
Potato starch processing waste is causing serious environmental problems. This study aimed to convert potato starch processing waste into single-cell protein as high-quality feed using a two-step fermentation process. The mutant strain Aspergillus niger H3 was selected after UV irradiation and ethyl methyl sulfone mutagenesis for more cellulase production. The activities of sodium carboxymethyl cellulase and filter paperase of strain H3 were 8.86 and 4.79 U, respectively, which were much higher than the parent strain (1.18 and 0.62 U). After treatment with strain H3, the cellulose degradation rate of potato residue was 80.54 %. A liquid fermentation using Bacillus licheniformis was performed as the second step. The optimized fermentation conditions were temperature of 32.8 A degrees C, pH 6.67, and inoculum concentration of 1.78 % using the response surface method. Results of this study showed a potential application in large-scale industrial conversion.
引用
收藏
页码:3637 / 3645
页数:9
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