New procedure for selective extraction of polycyclic aromatic hydrocarbons in plants for gas chromatographic-mass spectrometric analysis

被引：40
|
作者：
Dugay, A
Herrenknecht, C
Czok, M
Guyon, F
Pages, N
机构：
[1] Fac Pharm Chatenay Malabry, Chim Analyt Lab, F-92290 Chatenay Malabry, France
[2] Fac Pharm, Chim Analyt Lab, F-75005 Paris, France
[3] Fac Pharm, Chim Analyt Lab, F-37200 Tours, France
[4] Fac Pharm, Toxicol Lab, F-67400 Illkirch Graffenstaden, France
来源：
JOURNAL OF CHROMATOGRAPHY A | 2002年 / 958卷 / 1-2期
关键词：
solid-phase extraction; phytoremediation; polynuclear aromatic hydrocarbons;
D O I：
10.1016/S0021-9673(02)00383-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new solid-phase extraction method for the clean-up and the quantitation by GC-MS of regulated polycyclic aromatic hydrocarbons (PAHs) from lettuce was developed and the experimental conditions were optimized. After ultrasonic extraction using toluene and saponification of samples, a clean-up of extracts through solid-phase extraction was performed. Samples were finally analyzed by gas chromatography-mass spectrometry (GC-MS) using an internal deuterated standard. Saponification by KOH in methanol-water (80:20) was successful allowing a good elimination of the interfering chlorophylls from the extracts containing the PAHs. The average recovery of the 16 regulated PAHs was 70, 74, 79 and 89%, respectively, for naphthalene. acenaphthylene, acenaphthene and chrysene and higher than 94% for the others. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：1 / 7
页数：7
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