The aim of this work is to evaluate weed species diversity in spring barley on every variant of crop rotation. Evaluation was made on the field experiment in Zabcice. There were used three variants of crop rotations. Reached results were evaluated by CCA (Canonical Correspondence Analysis). On variant with monoculture of spring barley were mainly: Avena fatua, Stellaria media, Cirsium arvense, Microrrhinum minus, Silene noctiflora, Fallopia convolvulus, Sonchus oleraceus, Persicaria lapathifolia. On the second variant with Norfolk crop rotation appeared species: Viola arvensis, Lamium amplexicaule, Polygonum aviculare, Trifolium pretense. The third group of weeds appeared mainly on the variant where was used the 5th crop rotation and there were: Chenopodium album, Thlaspi arvense, Fumaria officinalis, Carthamus tinctorius.