Rapid diagnostics of Mycoplasma bovis infection in cattle by capture-ELISA-test and selective differentiating medium

The authors examined the usability of capture ELISA test and a selective differentiating medium in the diagnostics of the Mycoplasma bovis infection. Out of 52 strains, isolated from 510 various clinical specimens, 43 were proven to be M. bovis by both culturing and capture ELISA. Out of 92 lung specimen, 15 Mycoplasma strains were isolated. All of them were identified as M. bovis by the above mentioned diagnostic methods. Samples from M. bovis challenged animals were examined by culturing, selective culturing and capture ELISA. The two latter methods have been proven to be rapid, highly specific and useful in the diagnostics of M. bovis infection of cattle. Capture ELISA and selective-differentiating medium gives the same result as the time consuming conventional culturing of this organism.