Purification and partial characterization of an endo-(1->3,1->4)-beta-glucanase from rice, Oryza sativa L

被引：7

作者：

Akiyama, T ^{[1
]}

Kaku, H ^{[1
]}

Shibuya, N ^{[1
]}

机构：

[1] NATL INST AGROBIOL RESOURCES, DEPT CELL BIOL, TSUKUBA, IBARAKI 305, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 12期

关键词：

(1->3,1->4)-beta-glucanase; rice grain; Oryza sativa;

D O I：

10.1271/bbb.60.2078

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(1-->3,1-->4)-beta-Glucanase (EC 3.2.1.73), with a molecular weight of 34,000 and an isoelectric point of 4.9, was purified to homogeneity from extracts of fresh rice bran. The enzyme specifically hydrolyzed (1-->3,1-->4)-beta-glucans such as barley beta-glucan and lichenans, but laminarins and CM-cellulose were not substrates. Endproduct analysis using barley beta-glucan as the substrate suggested that the enzyme is an endo-type (1-->3,1-->4)-beta-glucanase.

引用

页码：2078 / 2080

页数：3

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