PF-4708671 Activates AMPK Independently of p70S6K1 Inhibition

被引：8
|
作者：
Vainer, Gilad W. [1 ]
Saada, Ann [2 ,3 ]
Kania-Almog, Juliane [1 ]
Amartely, Adir [4 ]
Bar-Tana, Jacob [5 ]
Hertz, Rachel [5 ]
机构：
[1] Tel Aviv Univ, Tel Aviv Sourasky Med Ctr, Dept Pathol, IL-69978 Tel Aviv, Israel
[2] Hadassah Hebrew Univ, Monique & Jacques Roboh Dept Genet Res, Med Ctr, Jerusalem, Israel
[3] Hadassah Hebrew Univ, Dept Genet & Metab Dis, Med Ctr, Jerusalem, Israel
[4] Hadassah Hebrew Univ, Dept Pathol, Med Ctr, Jerusalem, Israel
[5] Hebrew Univ Jerusalem, Sch Med, IL-91010 Jerusalem, Israel
来源：
PLOS ONE | 2014年 / 9卷 / 09期
关键词：
RIBOSOMAL-PROTEIN S6; CROSS-TALK; KINASE; MTOR; INSULIN;
D O I：
10.1371/journal.pone.0107364
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The P70 ribosomal protein S6 kinase 1 (P70S6K1) is activated by the mammalian target of rapamycin (mTORC1) and regulates proliferation, growth, and metabolism. PF-4708671 is a novel, cell-permeable, has been proposed to be a highly specific inhibitor of p70S6K1. It is used in micromolar concentration range to dissect signaling pathways downstream of mTORC1 and to study the function of p70S6K1. Here we show that PF-4708671 induces AMP-activated protein kinase (AMPK) phosphorylation and activation in immortalized mouse embryonic fibroblasts (MEF) independently of p70S6K1, due to specific inhibition of mitochondrial respiratory chain Complex I.
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页数：5
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