Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit

被引：24
|
作者：
Okitsu-Negishi, Shoko
Kame, Michio
Shimizu, Yuko
Phan, Tung Gia
Tomaru, Takeshi
Kamijo, Shigenori
Sato, Takashi
Yagyu, Fumihiro
Mueller, Werner E. G.
Ushijima, Hiroshi
机构：
[1] Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
[2] Nippon Becton Dickinson Co, Tokyo, Japan
[3] Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-6500 Mainz, Germany
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 10期
关键词：
D O I：
10.1128/JCM.01373-06
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
引用
收藏
页码：3784 / 3786
页数：3
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