Extraction of extracellular polymeric substances (EPS) from red soils (Ultisols)

被引：27
|
作者：
Wang, Shuang [1 ]
Redmile-Gordon, Marc [2 ]
Mortimer, Monika [3 ,4 ]
Cai, Peng [1 ]
Wu, Yichao [1 ]
Peacock, Caroline L. [5 ]
Gao, Chunhui [1 ]
Huang, Qiaoyun [1 ]
机构：
[1] Huazhong Agr Univ, Coll Resources & Environm, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
[2] Royal Hort Soc, Dept Environm Hort, Wisley GU23 6QB, Surrey, England
[3] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Earth Res Inst, Santa Barbara, CA 93106 USA
[4] Univ Calif Santa Barbara, UC CEIN, Santa Barbara, CA 93106 USA
[5] Univ Leeds, Sch Earth & Environm, Leeds LS2 9JT, W Yorkshire, England
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 2019年 / 135卷
基金：
中国国家自然科学基金;
关键词：
Soil biofilms; Extracellular polymeric substances; Polysaccharide; Cation exchange resin; Easily extractable glomalin; ACTIVATED-SLUDGE; ORGANIC-MATTER; EFFICIENCY; BIOFILM;
D O I：
10.1016/j.soilbio.2019.05.014
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Extracellular polymeric substances (EPS) have many beneficial functions in soils. Accurate quantification of EPS in soils is crucial. Here, five methods were compared for their suitability for extraction of EPS from Ultisols: hot water extractable polysaccharide (HWEP), hot dilute acid extractable polysaccharide (HDAEP), easily extractable glomalin (EEG), sodium sulfide (SS) and cation exchange resin (CER) method. Humic-acid equivalent (HAE) was used as an indicator for extracellular contamination and ATP for quantifying intracellular contamination from cell lysis. Among the tested methods, CER resulted in EPS extraction with minimal contamination. Therefore, we propose that CER is currently the most appropriate method for extraction of EPS from Ultisols.
引用
收藏
页码：283 / 285
页数：3
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