Polymorphic microsatellites in a mangrove species, Rhizophora mangle L. (Rhizophoraceae)

被引：26
|
作者：
Rosero-Galindo, C [1 ]
Gaitan-Solis, E
Cárdernas-Henao, H
Tohme, J
Toro-Perea, N
机构：
[1] Univ Valle Cali Valle, Dept Biol, Mol Biol Lab, Secc Genet, AA-25360 Cali, Colombia
[2] Ctr Int Agr Trop, CIAT, Unidad Biotecnol, Palmira, Valle, Colombia
来源：
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期
关键词：
enriched library; mangrove; microsatellite; Rhizophora mangle;
D O I：
10.1046/j.1471-8286.2002.00232.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Twenty-six microsatellite loci were isolated and characterized from the mangrove species Rhizophora mangle using (GT)(n) and (CT)(n) repeats. Eighty-four percent of the clones contained microsatellite sequences; the most common dinucleotides were the (GA/CT) and (CA/GT) repeats. Ten primers were selected to investigate the polymorphism among individuals of R. mangle from two natural populations of the Colombian Pacific Coast. The observed heterozygosity per locus varied from 0.20 to 0.80, the power of discrimination was 0.32-0.84 and the power of exclusion was 0.03-0.75. This set of microsatellites offers an efficient tool for population genetics studies on this species.
引用
收藏
页码：281 / 283
页数：3
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