Site-specific endonuclease BcuAI from Bacillus cereus A

被引：0
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作者：
Korolev, SV
Samko, OT
Eldarov, MA
Kalugin, AA
Khoroshutina, EB
Omelyanyuk, NM
Sokolov, NN
Skryabin, KG
机构：
[1] RUSSIAN ACAD MED SCI, INST BIOMED CHEM, MOSCOW 119832, RUSSIA
[2] RUSSIAN ACAD SCI, CTR BIOENGN, MOSCOW, RUSSIA
来源：
BIOORGANICHESKAYA KHIMIYA | 1996年 / 22卷 / 07期
关键词：
restriction endonucleases type II; restriction endonuclease BcuAI; AvaII isoschisomer; Bacillus cereus A;
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学科分类号：
摘要：
A new restriction endonuclease was isolated from the Bacillus cereus BKM B-814 by means of the cell disruption with ultrasonication, ammonium sulfate fractionation of the cell-free extract, and chromatography on DEAE-Sepharose to give about 1400 U of the enzyme per gram of cells. The enzyme revealed the maximum activity at 30-37 degrees C, pH 7.6-8.2, and 5-10 mM MgCl2 under a high ionic strength (50 mM Tris-HCl, 100 mM NaCl). The site-specific endonuclease BcuAI was found to recognize the 5' G down arrow G(A/T)CC sequence in double-stranded DNA and cleave it as shown with the arrow, thus being a true isoschisomer of the Avail restriction endonuclease.
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页码：528 / 531
页数：4
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