Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis

被引：51
|
作者：
Lemus, D
Martin, A
Montoro, E
Portaels, F
Palomino, JC
机构：
[1] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium
[2] Inst Med Trop Pedro Kouri, Havana, Cuba
来源：
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2004年 / 54卷 / 01期
关键词：
M; tuberculosis; MTT; resazurin; nitrate reductase assay; rifampicin;
D O I：
10.1093/jac/dkh320
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance. Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Lowenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit. Results: Complete agreement was observed among all methods. Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.
引用
收藏
页码：130 / 133
页数：4
相关论文
共 50 条
  • [1] Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium tuberculosis
    Lemus, Dihadenys
    Martin, Anandi
    Montoro, Ernesto
    Portaels, Franoise
    Palomino, Juan Carlos
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2005, 26 : S96 - S96
  • [2] Rifampicin resistance in Mycobacterium tuberculosis - Rapid detection and implications in chemotherapy
    Pretorius, GS
    Sirgel, FA
    Schaaf, HS
    vanHelden, PD
    Victor, TC
    [J]. SOUTH AFRICAN MEDICAL JOURNAL, 1996, 86 (01): : 50 - 55
  • [3] GeneXpert: A new tool for the rapid detection of rifampicin resistance in mycobacterium tuberculosis
    Saeed, Muhammad
    Iram, Shagufta
    Hussain, Shahida
    Ahmed, Adeel
    Akbar, Mamoon
    Aslam, Maleeha
    [J]. JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION, 2017, 67 (02) : 270 - 274
  • [4] GeneXpert: A new tool for the rapid detection of rifampicin resistance in mycobacterium tuberculosis
    Saeed, M.
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2020, 101 : 188 - 188
  • [5] Rifoligotyping assay: an alternative method for rapid detection of rifampicin resistance in Mycobacterium tuberculosis isolates from Morocco
    Chaoui, Imane
    Atalhi, Naima
    Sabouni, Radia
    Akrim, Mohammed
    Abid, Mohammed
    Amzazi, Saaid
    ElMzibri, Mohammed
    [J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2014, 28 (06) : 1095 - 1102
  • [6] Pyrosequencing for the rapid detection of rifampicin resistance in Mycobacterium tuberculosis: a meta-analysis
    Guo, Q.
    Zheng, R-J
    Zhu, C-T.
    Zou, L-L.
    Xiu, J-F.
    Li, J.
    Hu, Z-Y.
    [J]. INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2013, 17 (08) : 1008 - 1013
  • [7] Xpert MTB/RIF assay for rapid detection of Mycobacterium tuberculosis and rifampicin resistance
    Bhattacharyya, Debajyoti
    Agarwal, A.
    Barthwal, M. S.
    Katoch, C. D. S.
    [J]. EUROPEAN RESPIRATORY JOURNAL, 2012, 40
  • [8] Evaluation of Phage assay for rapid phenotypic detection of rifampicin resistance in Mycobacterium tuberculosis
    Yzquierdo S.L.
    Lemus D.
    Echemendia M.
    Montoro E.
    McNerney R.
    Martin A.
    Palomino J.C.
    [J]. Annals of Clinical Microbiology and Antimicrobials, 5 (1)
  • [9] Mechanisms and detection methods of Mycobacterium tuberculosis rifampicin resistance: The phenomenon of drug resistance is complex
    Xu, Ge
    Liu, Hangchi
    Jia, Xudong
    Wang, Xiaomin
    Xu, Peng
    [J]. TUBERCULOSIS, 2021, 128
  • [10] Rapid detection of resistance to rifampicin in Mycobacterium leprae
    Roche, PW
    Shrestha, N
    Thomas, A
    Honore, N
    Cole, ST
    [J]. LEPROSY REVIEW, 2000, 71 : S96 - S97
12345