A Glycosaminoglycan Based, Modular Tissue Scaffold System for Rapid Assembly of Perfusable, High Cell Density, Engineered Tissues

被引:54
|
作者
Tiruvannamalai-Annamalai, Ramkumar [1 ]
Armant, David Randall [2 ,3 ,4 ]
Matthew, Howard W. T. [1 ,5 ]
机构
[1] Wayne State Univ, Dept Biomed Engn, Detroit, MI 48202 USA
[2] Wayne State Univ, Dept Obstet, Detroit, MI USA
[3] Wayne State Univ, Dept Gynecol, Detroit, MI USA
[4] NICHHD, Program Reprod & Adult Endocrinol, NIH, Bethesda, MD 20892 USA
[5] Wayne State Univ, Dept Chem Engn & Mat Sci, Detroit, MI USA
来源
PLOS ONE | 2014年 / 9卷 / 01期
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
MESENCHYMAL STEM-CELLS; SMOOTH-MUSCLE-CELLS; VASCULAR ENDOTHELIAL-CELLS; HUMAN SKIN EQUIVALENT; ARTICULAR CHONDROCYTES; HEPARAN-SULFATE; BLOOD-VESSEL; IN-VITRO; ANGIOGENESIS; HEPATOCYTES;
D O I
10.1371/journal.pone.0084287
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The limited ability to vascularize and perfuse thick, cell-laden tissue constructs has hindered efforts to engineer complex tissues and organs, including liver, heart and kidney. The emerging field of modular tissue engineering aims to address this limitation by fabricating constructs from the bottom up, with the objective of recreating native tissue architecture and promoting extensive vascularization. In this paper, we report the elements of a simple yet efficient method for fabricating vascularized tissue constructs by fusing biodegradable microcapsules with tunable interior environments. Parenchymal cells of various types, (i.e. trophoblasts, vascular smooth muscle cells, hepatocytes) were suspended in glycosaminoglycan (GAG) solutions (4%/1.5% chondroitin sulfate/carboxymethyl cellulose, or 1.5 wt% hyaluronan) and encapsulated by forming chitosan-GAG polyelectrolyte complex membranes around droplets of the cell suspension. The interior capsule environment could be further tuned by blending collagen with or suspending microcarriers in the GAG solution These capsule modules were seeded externally with vascular endothelial cells (VEC), and subsequently fused into tissue constructs possessing VEC-lined, inter-capsule channels. The microcapsules supported high density growth achieving clinically significant cell densities. Fusion of the endothelialized, capsules generated three dimensional constructs with an embedded network of interconnected channels that enabled long-term perfusion culture of the construct. A prototype, engineered liver tissue, formed by fusion of hepatocyte-containing capsules exhibited urea synthesis rates and albumin synthesis rates comparable to standard collagen sandwich hepatocyte cultures. The capsule based, modular approach described here has the potential to allow rapid assembly of tissue constructs with clinically significant cell densities, uniform cell distribution, and endothelialized, perfusable channels.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Biofabrication of engineered tissues by 3D bioprinting of tissue specific, high cell density bioinks
    Jeon, Oju
    [J]. TISSUE ENGINEERING PART A, 2022, 28 : 15 - 15
  • [2] Culturing of Ventricle Cells at High Density and Construction of Engineered Cardiac Cell Sheets Without Scaffold
    Guo, Yono
    Zhang, Xi-zheng
    Wei, Yan
    Guo, Chun
    Li, Rui-xin
    Zeng, Qiang-cheng
    Zhang, Yan-jun
    [J]. INTERNATIONAL HEART JOURNAL, 2009, 50 (05) : 653 - 662
  • [3] Modular Inductive High-Density Cell Culture Systems for Engineering Complex Tissues
    Alsberg, E.
    [J]. TISSUE ENGINEERING PART A, 2015, 21 : S47 - S47
  • [4] Superficial and bulk compressive properties of cartilagelike tissues repaired with a scaffold-free, stem cell-based tissue engineered construct (TEC)
    Nansai, Ryosuke
    Nakamura, Norimasa
    Fujie, Hiromichi
    [J]. Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C, 2010, 76 (769): : 2340 - 2344
  • [5] Laser assisted bioprinting of engineered tissue with high cell density and microscale organization
    Guillotin, Bertrand
    Souquet, Agnes
    Catros, Sylvain
    Duocastella, Marti
    Pippenger, Benjamin
    Bellance, Severine
    Bareille, Reine
    Remy, Murielle
    Bordenave, Laurence
    Amedee, Joelle
    Guillemot, Fabien
    [J]. BIOMATERIALS, 2010, 31 (28) : 7250 - 7256
  • [6] Development of High-Cell-Density Tissue Method for Compressed Modular Bioactuator
    Nomura, Takuto
    Takeuchi, Masaru
    Kim, Eunhye
    Huang, Qiang
    Hasegawa, Yasuhisa
    Fukuda, Toshio
    [J]. MICROMACHINES, 2022, 13 (10)
  • [7] High-resolution 3D scaffold model for engineered tissue fabrication using a rapid prototyping technique
    P. Quadrani
    A. Pasini
    M. Mattioli-Belmonte
    C. Zannoni
    A. Tampieri
    E. Landi
    F. Giantomassi
    D. Natali
    F. Casali
    G. Biagini
    A. Tomei-Minardi
    [J]. Medical and Biological Engineering and Computing, 2005, 43 : 196 - 199
  • [8] High-resolution 3D scaffold model for engineered tissue fabrication using a rapid prototyping technique
    Quadrani, P
    Pasini, A
    Mattioli-Belmonte, M
    Zannoni, C
    Tampieri, A
    Landi, E
    Giantomassi, F
    Natali, D
    Casali, F
    Biagini, G
    Tomei-Minardi, A
    [J]. MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 2005, 43 (02) : 196 - 199
  • [9] The Possibility of Creation Tissue-engineered Structures with a Structured Internal Nanocarbon Scaffold in an Organic Matrix for Repairing Tissues of the Cardiovascular System
    Volkova, Maria A.
    Korneev, Yury A.
    Zhurbina, Natalia N.
    Gerasimenko, Alexander Yu
    Glukhova, Olga E.
    [J]. PROCEEDINGS OF THE 2018 IEEE CONFERENCE OF RUSSIAN YOUNG RESEARCHERS IN ELECTRICAL AND ELECTRONIC ENGINEERING (EICONRUS), 2018, : 1952 - 1956
  • [10] MECHANICAL STIMULATION OF SCAFFOLD-FREE, CELL-BASED SINGLE FIBERS FOR TISSUE ENGINEERED TENDON
    Schiele, Nathan R.
    Koppes, Ryan A.
    Corr, David T.
    [J]. PROCEEDINGS OF THE ASME SUMMER BIOENGINEERING CONFERENCE 2011, PTS A AND B, 2011, : 499 - 500