Guide RNA Design for CRISPR/Cas9-Mediated Potato Genome Editing

被引：11

作者：

Khromov, A. V. ^{[1
,2
]}

Gushchin, V. A. ^{[1
,2
]}

Timerbaev, V. I. ^{[1
,3
]}

Kalinina, N. O. ^{[1
,2
]}

Taliansky, M. E. ^{[1
,4
]}

Makarov, V. V. ^{[1
,2
]}

机构：

[1] OOO Doka Gene Technol, Rogachevo 141880, Moscow Oblast, Russia

[2] Moscow MV Lomonosov State Univ, Moscow 119991, Russia

[3] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[4] James Hutton Inst, Dundee DD2 5DA, Scotland

来源：

DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2018年 / 479卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

CAS9;

D O I：

10.1134/S1607672918020084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of the pool of sgRNA molecules designed for different regions of potato coilin and phytoene desaturase genes was compared in vitro. Due to the presence of nucleotides unpaired with DNA, sgRNA is able not only to inhibit but also to stimulate the activity of the Cas9-sgRNA complex in vitro. Although the first six nucleotides located in the DNA substrate proximally to the PAM site at the 3' end are the binding sites for cas9, they had no significant effect on the activity of the Cas9-sgRNA complex.

引用

页码：90 / 94

页数：5

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