Lentisphaera profundi sp nov., isolated from deep-sea water

A Gram-staining-negative, aerobic, non-motile, coccus-shaped bacterium, designated SAORIC-696(T), was isolated from deep-sea water at a depth of 1700 m in the western North Pacific Ocean. Optimal growth of strain SAORIC-696(T) was observed at 15 degrees C, pH 7.0 and in the presence of 2 % (w/v) NaCl. Strain SAORIC-696(T) formed a robust phylogenetic clade with members of the genus Lentisphaera. The 16S rRNA gene sequence similarity showed that strain SAORIC-696(T) was most closely related to Lentisphaera marina (98.0 % similarity) and Lentisphaera araneosa (97.3 %). The DNA-DNA relatedness between SAORIC-696(T) and two species of the genus Lentisphaera was only 27-42 %. The DNA G+C content of strain SAORIC-696(T) was 43.1 mol% and predominant cellular fatty acids were C-16 : 1 omega 9c (36.8 %), C-14 : 0 (22.5 %) and C-14 : 0 3-OH and/or iso-C-16 : 1 vertical bar (10.8 %). Strain SAORIC-696(T) contained MK-7 as the only respiratory quinone. On the basis of taxonomic data collected in this study, it was concluded that strain SAORIC-696(T) represents a novel species of the genus Lentisphaera, for which the name Lentisphaera profundi sp. nov. is proposed, with the type strain SAORIC-696(T) (=NBRC 110692(T) = KCTC 42681(T)).