Src-mediated coupling of focal adhesion kinase to integrin αvβ5 in vascular endothelial growth factor signaling

Vascular endothelial growth factor (VEGF) promotes vascular permeability (VP) and neovascularization, and is required for development. We find that VEGF-stimulated Src activity in chick embryo blood vessels induces the coupling of focal adhesion kinase (FAK) to integrin alphavbeta5, a critical event in VEGF-mediated signaling and biological responsiveness. In contrast, FAK is constitutively associated with beta1 and beta3 integrins in the presence or absence of growth factors. In cultured endothelial cells, VEGF, but not basic fibroblast growth factor, promotes the Src-mediated phosphorylation of FAK on tyrosine 861, which contributes to the formation of a FAK/alphavbeta5 signaling complex. Moreover, formation of this FAK/alphavbeta5 complex is significantly reduced in pp60(C-SrC)-deficient mice. Supporting these results, mice deficient in either pp60(C-SrC), or integrin beta5, but not integrin beta3, have a reduced VP response to VEGF. This FAK/alphavbeta5 complex was also detected in epidermal growth factor-stimulated epithelial cells, suggesting a function for this complex outside the endothelium. Our findings indicate that Src can coordinate specific growth factor and extracellular matrix inputs by recruiting integrin alphavbeta5 into a FAK-containing signaling complex during growth factor-mediated biological responses.