In Vitro Chemotherapy of Pear Cultivars

被引：0
|
作者：
Paprstein, F. [1 ]
Sedlak, J. [1 ]
Talacko, L. [1 ]
机构：
[1] Res & Breeding Inst Pomol, Holovousy 50801, Horice, Czech Republic
来源：
II INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF FRUIT SPECIES | 2014年 / 1048卷
关键词：
ASPV; ELISA; PCR; virus detection; virus elimination; virus infection; STEM GROOVING VIRUS; RT-PCR; APPLE; ELIMINATION; CULTURES; ASSAYS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro plants of infected pear cultivars 'Astra', 'David' and 'Erika'. According to RT-PCR testing, selected field-grown trees of all pear cultivars were infected with ASPV. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was used at 20 mg L-1 for virus elimination. Low phytotoxicity of ribavirin at 20 mg L-1 was observed only for the cultivar 'David'. Six of the twenty shoot tips of 'David' showed apex necrosis and died after transfer to regeneration medium. The success of chemotherapy after RT-PCR testing of ribavirin treated plants varied between 65.0% for cultivars 'Astra' and 'Erika' and 71.4% for cultivar 'David'. The results achieved and the relatively high percentage of virus free plants obtained after 6 months of in vitro cultivation confirm the effectiveness of ribavirin in eliminating ASPV in pear.
引用
收藏
页码：221 / 224
页数：4
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