Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo

被引：7

作者：

Knipes, Alaine K. ^{[1
,14
]}

Summers, Aimee ^{[1
,13
]}

Sklavounos, Alexandros A. ^{[2
,3
]}

Lamanna, Julian ^{[2
,3
]}

de Campos, Richard P. S. ^{[2
,3
,4
]}

Narahari, Tanya ^{[2
,3
]}

Dixon, Christopher ^{[2
]}

Fobel, Ryan ^{[2
,5
]}

Ndjakani, Yassa D. ^{[6
]}

Lubula, Leopold ^{[7
]}

Magazani, Alain ^{[7
]}

Muyembe, Jean Jacques ^{[8
]}

Lay, Yvonne ^{[8
]}

Pukuta, Elizabeth ^{[8
]}

Waku-Kouomou, Diane ^{[9
]}

Hao, Lijuan ^{[9
]}

Kayembe, Jolie Kasongo ^{[10
]}

Fobel, Christian ^{[2
,5
]}

Dahmer, Joshua ^{[2
,3
]}

Lee, Adam ^{[2
]}

Ho, Man ^{[2
]}

Valenzuela, Jose Gilberto Camacho ^{[3
,11
]}

Rackus, Darius G. ^{[2
,3
]}

Shih, Roger ^{[2
,3
]}

Seale, Brendon ^{[2
]}

Chang, Ann ^{[2
]}

Paluku, Gilson ^{[12
]}

Rota, Paul A. ^{[13
]}

Wheeler, Aaron R. ^{[2
,3
,11
]}

Scobie, Heather M. ^{[13
]}

机构：

[1] Ctr Dis Control & Prevent, Div Global Hlth Protect, Ctr Global Hlth, Atlanta, GA 30329 USA

[2] Univ Toronto, Dept Chem, Toronto, ON, Canada

[3] Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON, Canada

[4] Natl Res Council Canada, Nanotechnol Res Ctr, Edmonton, AB, Canada

[5] Sci Bots Inc, Kitchener, ON, Canada

[6] Ctr Dis Control & Prevent, Field Epidemiol & Lab Training Program, Ctr Global Hlth, Kinshasa, DEM REP CONGO

[7] Minist Hlth, Direct Gen Lutte Maladie, Kinshasa, DEM REP CONGO

[8] Minist Hlth, Inst Natl Rech Biomed, Kinshasa, DEM REP CONGO

[9] Ctr Dis Control & Prevent, Div Viral Dis, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USA

[10] Minist Hlth, Programme Elargi Vaccinat, Kinshasa, DEM REP CONGO

[11] Univ Toronto, Inst Biomed Engn, Toronto, ON, Canada

[12] WHO, Routine Immunizat & New Vaccine Intro, Libreville, Gabon

[13] Ctr Dis Control & Prevent, Global Immunizat Div, Ctr Global Hlth, Atlanta, GA USA

[14] Ctr Dis Control & Prevent, Div Parasit Dis & Malaria, Ctr Global Hlth, Atlanta, GA 30329 USA

来源：

PLOS ONE | 2022年 / 17卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CHILDREN; 6-59; MONTHS; GLOBAL MEASLES; ELIMINATION; WORLDWIDE; PROGRESS; AGE;

D O I：

10.1371/journal.pone.0278749

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Democratic Republic of the Congo (DRC) has a high measles incidence despite elimination efforts and has yet to introduce rubella vaccine. We evaluated the performance of a prototype rapid digital microfluidics powered (DMF) enzyme-linked immunoassay (ELISA) assessing measles and rubella infection, by testing for immunoglobulin M (IgM), and immunity from natural infection or vaccine, by testing immunoglobulin G (IgG), in outbreak settings. Field evaluations were conducted during September 2017, in Kinshasa province, DRC. Blood specimens were collected during an outbreak investigation of suspected measles cases and tested for measles and rubella IgM and IgG using the DMF-ELISA in the field. Simultaneously, a household serosurvey for measles and rubella IgG was conducted in a recently confirmed measles outbreak area. DMF-ELISA results were compared with reference ELISA results tested at DRC's National Public Health Laboratory and the US Centers for Disease Control and Prevention. Of 157 suspected measles cases, rubella IgM was detected in 54% while measles IgM was detected in 13%. Measles IgG-positive cases were higher among vaccinated persons (87%) than unvaccinated persons (72%). In the recent measles outbreak area, measles IgG seroprevalence was 93% overall, while rubella seroprevalence was lower for children (77%) than women (98%). Compared with reference ELISA, DMF-ELISA sensitivity and specificity were 82% and 78% for measles IgG; 88% and 89% for measles IgM; 85% and 85% for rubella IgG; and 81% and 83% for rubella IgM, respectively. Rubella infection was detected in more than half of persons meeting the suspected measles case definition during a presumed measles outbreak, suggesting substantial unrecognized rubella incidence, and highlighting the need for rubella vaccine introduction into the national schedule. The performance of the DMF-ELISA suggested that this technology can be used to develop rapid diagnostic tests for measles and rubella.

引用

页数：14

共 3 条

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Alberti, Kathryn P.
King, Lisa A.
Burny, Marie-Eve
Ilunga, Benoit Kebela
Grais, Rebecca F.
INTERNATIONAL HEALTH, 2010, 2 (01): : 65 - 68
[2] Estimating the burden of rubella virus infection and congenital rubella syndrome through a rubella immunity assessment among pregnant women in the Democratic Republic of the Congo: Potential impact on vaccination policy
Alleman, Mary M.
Wannemuehler, Kathleen A.
Hao, Lijuan
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VACCINE, 2016, 34 (51) : 6502 - 6511
[3] Factors Contributing to Outbreaks of Wild Poliovirus Type 1 Infection Involving Persons Aged ≥15 Years in the Democratic Republic of the Congo, 2010-2011, Informed by a Pre-Outbreak Poliovirus Immunity Assessment
Alleman, Mary M.
Wannemuehler, Kathleen A.
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Mulumba, Audry
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Ntumbannji, Renee N.
Coulibaly, Tiekoura
Abiola, Nadine
Mpingulu, Minlangu
Sidibe, Kassim
Oberste, M. Steven
JOURNAL OF INFECTIOUS DISEASES, 2014, 210 : S62 - S73

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