Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization

被引：30

作者：

Tanaka, Hiroyuki ^{[1
,2
]}

Hanasaki, Minako ^{[2
]}

Isojima, Tatsushi ^{[2
]}

Takeuchi, Hisao ^{[2
]}

Shiroya, Toshifumi ^{[2
]}

Kawaguchi, Haruma ^{[1
]}

机构：

[1] Keio Univ, Grad Sch Sci & Technol, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

[2] Mitsubishi Chem Grp Sci & Technol INC, Aoba Ku, Yokohama, Kanagawa 2278502, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 70卷 / 02期

关键词：

Protein microarray; Hydrogel; Protein immobilization; SPR biosensor; Reactive polymer; GOLD SURFACES; TECHNOLOGY; HYDROGEL; BINDING; MATRIX; ARRAYS;

D O I：

10.1016/j.colsurfb.2008.12.037

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We discovered a novel method to prepare a protein-based hydrogel, that is, a "Three-Dimensional Nanostructured Protein Hydrogel (3D NPH)", which is composed of protein-polymer hybrid nanoparticles. In this study, we propose a novel protein microarray whose 3D NPH spots were prepared by dispensing a small volume of the Solution of protein-polynier mixture on a substrate. The dispensed solution had a short time for cross-linking before its drying-up and the resulting 3D NPH had loosely cross-linked, thin spongy structure. Therefore, the reaction ratio between ligands and analytes was drastically improved in this system compared with the large volume system for Surface Plasmon Resonance (SPR) protein microarray. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：259 / 265

页数：7

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