Effect of Formic Acid and Inoculants on Microbial Community and Fermentation Profile of Wilted or Un-Wilted Italian Ryegrass Silages during Ensiling and Aerobic Exposure

被引:4
|
作者
Ran, Qifan [1 ]
Guan, Hao [2 ]
Li, Haiping [3 ]
He, Wei [1 ]
Zhu, Ruifen [1 ]
Zhang, Li [1 ]
Huang, Yong [1 ]
Xu, Yuandong [1 ]
Fan, Yan [1 ]
机构
[1] Chongqing Acad Anim Sci, Inst Grassland Sci, Chongqing 402460, Peoples R China
[2] Southwest Minzu Univ, Sichuan Zoige Alpine Wetland Ecosyst Natl Observat, Chengdu 610041, Peoples R China
[3] Qinghai Normal Univ, Sch Math & Stat, Xining 810016, Peoples R China
来源
FERMENTATION-BASEL | 2022年 / 8卷 / 12期
关键词
silage; microbial community; fermentation profile; aerobic stability; Italian ryegrass; LACTOBACILLUS-PLANTARUM; LACTIC-ACID; BUNKER-SILO; ALFALFA; CORN; STABILITY; BACTERIAL; BUCHNERI; DRY; ADDITIVES;
D O I
10.3390/fermentation8120755
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Wilted (around 35% DM) or un-wilted (around 35% DM) Italian ryegrass treated with three additives (formic acid, FA; Lactobacillus plantarum, LP; Lactobacillus buchneri, LB) was utilized to evaluate the effects of the dry matter (DM) contents on the microbial community and fermentation characteristics, which was ensiled for 60 days in a laboratory-scale silo, followed by 3 days of aerobic exposure. Significantly lower pH and higher lactic acid (LA) contents were observed in the LP-treated group ensiled at both DM contents (differences were significant when p < 0.05). The contents of LA, acetic acid (AA), numbers of lactic acid bacteria (LAB) and ammonia nitrogen (NH3-N) in the FA-treated group were significantly lower than those in other treatments (p < 0.05). L. buchneri was the dominant bacteria after 60 days fermentation, while Enterobacteria became prevalent after 3 days of aerobic exposure. L. buchneri was found in the LB-treated group with higher acetic acid. Although the best fermentation quality was observed in the LP-treated silages, the aerobic stability was lowest compared to other groups (p < 0.05). In conclusion, our findings suggest that the DM content of Italian ryegrass affected its epiphytic microbial community and the effectiveness of the different type of additives. Formic acid was more suitable for un-wilted Italian ryegrass silage, L. plantarum had a better effect in wilted Italian ryegrass silage, and L. buchneri prolonged the aerobic stability of Italian ryegrass. DM content and purpose of ensiling should be the key factors for choosing different types of additives for Italian ryegrass silage.
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页数:14
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