Real-time fluorescence and deformability cytometry

被引：115

作者：

Rosendahl, Philipp ^{[1
]}

Plak, Katarzyna ^{[1
,2
]}

Jacobi, Angela ^{[1
]}

Kraeter, Martin ^{[1
,3
]}

Toepfner, Nicole ^{[1
,4
]}

Otto, Oliver ^{[1
]}

Herold, Christoph ^{[1
]}

Winzi, Maria ^{[1
]}

Herbig, Maik ^{[1
]}

Ge, Yan ^{[1
]}

Girardo, Salvatore ^{[5
]}

Wagner, Katrin ^{[1
]}

Baum, Buzz ^{[2
]}

Guck, Jochen ^{[1
]}

机构：

[1] Tech Univ Dresden, Ctr Biotechnol, Ctr Mol & Cellular Bioengn, Dresden, Germany

[2] UCL, MRC Lab Mol & Cellular Biol, London, England

[3] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Med Clin 1, Dresden, Germany

[4] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Dept Pediat, Dresden, Germany

[5] Tech Univ Dresden, Ctr Mol & Cellular Bioengn, Microstruct Facil, Dresden, Germany

来源：

NATURE METHODS | 2018年 / 15卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

MECHANICS; CELLS;

D O I：

10.1038/nmeth.4639

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.

引用

页码：355 / +

页数：7

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