Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system

被引：0
|
作者：
Baltes, Nicholas J. [1 ,2 ]
Hummel, Aaron W. [1 ,2 ]
Konecna, Eva [1 ,2 ]
Cegan, Radim [3 ]
Bruns, Aaron N. [4 ,5 ]
Bisaro, David M. [4 ,5 ]
Voytas, Daniel F. [1 ,2 ]
机构：
[1] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Ctr Genome Engn, Minneapolis, MN 55455 USA
[3] Inst Biophys ASCR, Dept Plant Dev Genet, Vvi, Brno 61200, Czech Republic
[4] Ohio State Univ, Ctr Appl Plant Sci, Dept Mol Genet, Columbus, OH 43210 USA
[5] Ohio State Univ, Ohio State Biochem Program, Columbus, OH 43210 USA
来源：
NATURE PLANTS | 2015年 / 1卷 / 10期
基金：
美国国家科学基金会;
关键词：
SEQUENCE; PLANT; RNA; DNA; PLATFORM;
D O I：
10.1038/NPLANTS.2015.145
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.
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页数：4
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