Electrochemistry and electrocatalytic activity of pyrocatechol violet (PCV) film on a glassy carbon electrode towards the oxidation of reduced nicotinamide adenine dinucleotide(NADH)

Electrocatalytic oxidation of nicotinamide adenine dinucleotied (NADH) at a glassy carbon electrode modified with electrodeposited film derived from pyrocatechol violet (PCV) has been studied. The PCV modified glassy carbon electrode shows excellent electrocatalytic activity toward NADH oxidation in phosphate buffer solution (pH 7.0) with an overpotential of about 447 mV lower than that of the bare electrode. The apparent charge transfer rate constant and charge transfer coefficient for electron transfer between the electrode surface and immobilized PCV were calculated as 5.3 s(-1) and 0.49, respectively. The catalytic rate constant of the modified glassy carbon electrode for the oxidation of NADH is determined by cyclic voltammetry and rotating disk electrode measurements. Also, it has been shown that using the PCV modified electrode, NADH can be estimated by amperometry with high sensitivity and good limit of detection.