Human sebocytes express prostaglandin E2 receptors EP2 and EP4 but treatment with prostaglandin E2 does not affect testosterone production

被引:13
|
作者
Chen, W. [2 ,3 ]
Tsai, S. -J. [1 ]
Wang, C. -A. [1 ]
Tsai, J. -C. [1 ]
Zouboulis, C. C. [4 ,5 ,6 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Physiol, Tainan 70101, Taiwan
[2] Chang Gung Univ, Dept Dermatol, Chang Gung Mem Hosp, Kaohsiung Med Ctr, Kaohsiung, Taiwan
[3] Tech Univ Munich, Dept Dermatol & Allergy, Munich, Germany
[4] Dessau Med Ctr, Dept Dermatol, Dessau, Germany
[5] Dessau Med Ctr, Dept Venereol, Dessau, Germany
[6] Dessau Med Ctr, Dept Allergol & Immunol, Dessau, Germany
关键词
acne; prostaglandin E2; prostanoid receptors; sebaceous glands; testosterone; ENDOMETRIOTIC STROMAL CELLS; ACUTE REGULATORY PROTEIN; SEBACEOUS GLANDS; E-2; PRODUCTION; SZ95; SKIN;
D O I
10.1111/j.1365-2133.2009.09165.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background Prostaglandins (PG) play an important role in cutaneous homeostasis. Among other skin cells, human sebocytes express cyclooxygenases and can produce PGE(2). Various prostanoid receptors have been demonstrated in epidermis and hair follicles, while limited data are available regarding their expression in sebaceous glands. In addition, the interaction between PGE(2) and androgenesis remains largely unclear. Objectives To examine the expression of PGE(2) receptor (EP) and PGF(2 alpha) receptor (FP) in human sebocytes and the influence of PGE(2) or PGF(2 alpha) on testosterone production. Methods A reverse transcription-polymerase chain reaction study was used to detect the expression of EP subtypes and FP. A testosterone radioimmunoassay was used to measure the amount of testosterone in the supernatant of cultured SZ95 sebocytes treated with PGE(2) or PGF(2 alpha) alone or in the presence of various androgen precursor substrates. Results SZ95 sebocytes expressed mainly EP2 and EP4 but not EP3 or FP. Testosterone production was not induced by PGE(2) or PGF(2 alpha), alone or in the presence of cholesterol. PGE(2) did not affect androgenesis in cultured sebocytes. Conclusions The expression patterns of prostanoid receptors differ between sebocytes, hair follicles and epidermis. The effects of PGE(2) and PGF(2 alpha) on the proliferation, lipogenesis and inflammation of sebocytes appear not to be associated with androgenesis.
引用
收藏
页码:674 / 677
页数:4
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