In vitro bioactive potential and chemical analysis of the n-hexane extract of the medicinal mushroom, Cordyceps militaris

被引:3
|
作者
EI-Hagrassi, Ali [1 ]
Daba, Ghoson [2 ]
Elkateeb, Waill [2 ]
Ahmed, Eman [2 ]
El-Dein, Asmaa Negm [2 ]
Fayad, Walid [3 ]
Shaheen, Mohamed [4 ]
Shehata, Riham [4 ]
El-Manawaty, May [3 ]
Wen, Ting-Chi [5 ]
机构
[1] Natl Res Ctr, Pharmaceut Ind Div, Phytochem & Plant Systemat Dept, Giza 12622, Egypt
[2] Natl Res Ctr, Chem Nat & Microbial Prod Dept, Pharmaceut Ind Div, Giza 12622, Egypt
[3] Natl Res Ctr, Pharmacognosy Dept, Drug Bioassay Cell Culture Lab, Giza 12622, Egypt
[4] Natl Res Ctr, Water Pollut Res Dept, Environm Virol Lab, Environm Res Div, Cairo, Egypt
[5] Guizhou Univ, Minist Educ, Engn Res Ctr Southwest Biopharmaceut Resources, Guiyang 550025, Guizhou, Peoples R China
关键词
Medicinal mushroom; Cordyceps militaris; antiviral; GC-MS analysis; fatty acids; POLYSACCHARIDES; INFLAMMATION; BODY;
D O I
10.21161/mjm.190346
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Aims: Cordyceps militaris is a medicinal mushroom from Ascomycota. The aims of this study were to explore and identify the chemical compounds extracted in the non-polar fraction of the mushroom and to examine the biological potential of this extract. Methodology and results: The n-hexane extract metabolites were identified using gas chromatography-mass spectrometry (GC-MS) and results revealed the presence of 37 compounds delivered from different chemical classes and were mainly comprised of fatty acids and their esters (72%), carboxylic acids and their esters (10.39%), and a sulphur compound (7.1%). The n-hexane extract recorded a promising antioxidant effect (80.9 +/- 1.5%) at 80 mg/mL total extract; potent cholesterol reduction activity (100%) was obtained after 96 h incubation by the total metabolites (4%). The cytotoxicity of the compounds revealed 50% cytotoxicity concentration (CC50). 1 mg/mL and anti-rotavirus SA-11 effect where inhibition of virus attachment and penetration into infected cells was recorded at 50% effective concentration (IC50) of 300 +/- 0.2 mu g/mL. Conclusion, significance and impact of study: This study confirmed the impact of the fatty acids produced by C. militaris as bioactive metabolites.
引用
收藏
页码:40 / 48
页数:9
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