Cooperative binding of the outer arm-docking complex underlies the regular arrangement of outer arm dynein in the axoneme

被引:38
|
作者
Owa, Mikito [1 ]
Furuta, Akane [1 ]
Usukura, Jiro [2 ]
Arisaka, Fumio [3 ]
King, Stephen M. [4 ]
Witman, George B. [5 ]
Kamiya, Ritsu [1 ,6 ]
Wakabayashi, Ken-ichi [7 ]
机构
[1] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan
[2] Nagoya Univ, EcoTopia Sci Inst, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[3] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Yokohama, Kanagawa 2268510, Japan
[4] Univ Connecticut, Dept Mol Biol & Biophys, Ctr Hlth, Farmington, CT 06030 USA
[5] Univ Massachusetts, Dept Cell & Dev Biol, Sch Med, Worcester, MA 01655 USA
[6] Gakushuin Univ, Fac Sci, Dept Life Sci, Tokyo 1718588, Japan
[7] Tokyo Inst Technol, Chem Resources Lab, Yokohama, Kanagawa 2268503, Japan
基金
美国国家卫生研究院;
关键词
INTERMEDIATE CHAIN; SUBUNIT; PROTEIN; FLAGELLA; CILIA; IDENTIFICATION; ARCHITECTURE; MUTATIONS; TRANSPORT; MUTANTS;
D O I
10.1073/pnas.1403101111
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Outer arm dynein (OAD) in cilia and flagella is bound to the outer doublet microtubules every 24 nm. Periodic binding of OADs at specific sites is important for efficient cilia/flagella beating; however, the molecular mechanism that specifies OAD arrangement remains elusive. Studies using the green alga Chlamydomonas reinhardtii have shown that the OAD-docking complex (ODA-DC), a heterotrimeric complex present at the OAD base, functions as the OAD docking site on the doublet. We find that the ODA-DC has an ellipsoidal shape similar to 24 nm in length. In mutant axonemes that lack OAD but retain the ODA-DC, ODA-DC molecules are aligned in an end-to-end manner along the outer doublets. When flagella of a mutant lacking ODA-DCs are supplied with ODA-DCs upon gamete fusion, ODA-DC molecules first bind to the mutant axonemes in the proximal region, and the occupied region gradually extends toward the tip, followed by binding of OADs. This and other results indicate that a cooperative association of the ODA-DC underlies its function as the OAD-docking site and is the determinant of the 24-nm periodicity.
引用
收藏
页码:9461 / 9466
页数:6
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