Effects of the phosphatase inhibitors, okadaic acid, ATPγS, and calyculin A on the dividing sand dollar egg

被引:1
|
作者
Hamaguchi, Y
Kuriyama, R
机构
[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Bioengn, Meguro Ku, Tokyo 1528551, Japan
[2] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
关键词
actin; chromosome movement; cleavage furrow; microtubule; phosphatase inhibitor; sand dollar egg;
D O I
10.1247/csf.27.127
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effects of the phosphatase inhibitors, okadaic acid (OA), adenosine 5'-O-(3-thiotriphosphate) (ATPgammaS), and calyculin A (CL-A) on anaphase chromosome movement, cytokinesis, and cytoskeletal structures at cell division were examined by being microinjected into mitotic sand dollar eggs. When OA was injected, chromosome movement was inhibited and, moreover, chromosomes were ejected from the polar regions of the mitotic apparatus. By immunofluorescence, microtubules were observed to be severed in the OA-injected eggs, causing the smooth cell surface to be changed to an irregular surface. When ATPgammaS and CL-A were injected, the effect on cell shape was remarkable: In dividing eggs, furrowing stopped within several seconds after injection, small blebs appeared on the cell surface and became large, spherical or dumbbell cell shapes then changed to irregular forms, and subsequently cytoplasmic flow occurred. Microfilament detection revealed that actin accumulation in the cortex, which was not limited to the furrow cortex, occurred shortly after injection. Cortical accumulation of actin is thought to induce force generation and random cortical contraction, and accordingly to result in bleb extrusion from the cortex. Consequently, the phosphatase inhibitors inhibited the transition from mitosis to interphase by mediating cortical accumulation of actin filaments and/or fragmentation of microtubules.
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页码:127 / 137
页数:11
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