Proteus simulation software is different with other commonly used electronic simulation software, which is suitable for teaching and experiment courses. Proteus Software can be used to simulation analysis many electronic information professional courses,the circuit analysis, digital electronic circuit, analog electronic circuit, micro computer and embedded system, and the application in the experiment teaching, curriculum design and curriculum setting of electronic information be discussed. Through the Proteus simulation software to establish the virtual experiment platform help students theoretical knowledge understanding and practical skills training, can stimulate students' interest in learning, improve the students' ability of comprehensive design, to improve the teaching effect.