INCREASED VIABILITY OF PROBIOTIC LACTOBACILLUS RHAMNOSUS AFTER OSMOTIC STRESS

被引:14
|
作者
Wasko, A. [1 ]
Polak-Berecka, M. [1 ]
Gustaw, W. [2 ]
机构
[1] Univ Life Sci Lublin, Dept Biotechnol Human Nutr & Sci Food Commod, PL-20704 Lublin, Poland
[2] Univ Life Sci Lublin, Dept Fruit Vegetables & Fungi Technol, PL-20704 Lublin, Poland
关键词
Lactobacillus rhamnosus; freeze-drying; osmotic stress; shock proteins; 2-D electrophoresis; LACTIC-ACID BACTERIA; ENVIRONMENTAL-STRESS; ESCHERICHIA-COLI; SURVIVAL; ACIDOPHILUS; RESPONSES; RESISTANCE; TOLERANCE; PROTEINS; STORAGE;
D O I
10.1556/AAlim.42.2013.4.7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The objective of this work was to assess the impact of various NaCl concentrations on Lactobacillus rhamnosus OXY viability after freeze-drying. Osmotic stress was applied during the exponential growth phase of bacterial culture. At salt concentrations between 0.2-0.5 M, a high biomass concentration and a significant increase in cell viability after lyophilisation was observed. An analysis of two-dimensional protein gels indicated the presence of shock proteins, for example, GroEL, ClpB, DnaK, TF, which provide resistance during freeze-drying and subsequent storage. On the basis of these results, it is recommended that lactic acid bacteria cultures be sub-lethally treated with 0.5 M NaCl before freeze-drying.
引用
收藏
页码:520 / 528
页数:9
相关论文
共 50 条
  • [1] Heat and osmotic stress responses of probiotic Lactobacillus rhamnosus HN001 (DR20) in relation to viability after drying
    Prasad, J
    McJarrow, P
    Gopal, P
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) : 917 - 925
  • [2] Viability of probiotic Lactobacillus rhamnosus in structured emulsions containing saturated monoglycerides
    Marino, Marilena
    Innocente, Nadia
    Calligaris, Sonia
    Maifreni, Michela
    Mararigone, Anna
    Nicoli, Maria Cristina
    [J]. JOURNAL OF FUNCTIONAL FOODS, 2017, 35 : 51 - 59
  • [3] IMPACT OF SACCHAROMYCES CEREVISIAE ON VIABILITY OF PROBIOTIC LACTOBACILLUS RHAMNOSUS IN FERMENTED MILK UNDER AMBIENT CONDITIONS
    Suharja, Anggita A. S.
    Henriksson, Anders
    Liu, Shao-Quan
    [J]. JOURNAL OF FOOD PROCESSING AND PRESERVATION, 2014, 38 (01) : 326 - 337
  • [4] Proteomics and Transcriptomics Characterization of Bile Stress Response in Probiotic Lactobacillus rhamnosus GG
    Koskenniemi, Kerttu
    Laakso, Kati
    Koponen, Johanna
    Kankainen, Matti
    Greco, Dario
    Auvinen, Petri
    Savijoki, Kirsi
    Nyman, Tuula A.
    Surakka, Anu
    Salusjarvi, Tuomas
    de Vos, Willem M.
    Tynkkynen, Soile
    Kalkkinen, Nisse
    Varmanen, Pekka
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2011, 10 (02)
  • [5] Adaptation factors of the probiotic Lactobacillus rhamnosus GG
    Lebeer, S.
    Vanderleyden, J.
    De Keersmaecker, S. C. J.
    [J]. BENEFICIAL MICROBES, 2010, 1 (04) : 335 - 342
  • [6] Importance of biofilm age and growth medium on the viability of probiotic capsules containing Lactobacillus rhamnosus GG biofilm
    Kiew, Tie Yi
    Cheow, Wean Sin
    Hadinoto, Kunn
    [J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2014, 59 (02) : 956 - 963
  • [7] The study on the viability of lactobacillus rhamnosus in the gastrointestinal tract
    [J]. Li, Xiaodong (hrblxd@163.com), 1600, Chinese Institute of Food Science and Technology (16):
  • [8] Improvement of probiotic viability through the design of novel biomaterials using coffee pulp wastes and Lactobacillus rhamnosus
    Rosales Delgado, Stephania
    Alzate Arbelaez, Andres Felipe
    Zapata Acosta, Karol
    Cortes, Farid B.
    Rojano, Benjamin A.
    [J]. FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL, 2023, 29 (06) : 573 - 585
  • [9] Enhancing the viability of Lactobacillus rhamnosus GG after spray drying and during storage
    Broeckx, Geraldine
    Vandenheuvel, Dieter
    Henkens, Tim
    Kiekens, Shari
    van den Broek, Marianne F. L.
    Lebeer, Sarah
    Kiekens, Filip
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2017, 534 (1-2) : 35 - 41
  • [10] Preservation by freezing of potentially probiotic strains of Lactobacillus rhamnosus
    Succi, Mariantonietta
    Tremonte, Patrizio
    Reale, Anna
    Sorrentino, Elena
    Coppola, Raffaele
    [J]. ANNALS OF MICROBIOLOGY, 2007, 57 (04) : 537 - 544