A stop-flow comprehensive two-dimensional HK-2 and HK-2/CIKI cell membrane chromatography comparative analysis system for screening the active ingredients from Pyrrosia calvata (Bak.) Ching against crystal-induced kidney injury

被引：13

作者：

Pan, Pengchao ^{[1
]}

Cheng, Jin ^{[2
]}

Si, Yachen ^{[2
]}

Chen, Wei ^{[2
]}

Hou, Jiebin ^{[3
]}

Zhao, Tingting ^{[2
]}

Gu, Yanqiu ^{[4
]}

Lv, Lei ^{[5
]}

Hong, Zhanying ^{[1
]}

Zhu, Zhenyu ^{[1
]}

Chai, Yifeng ^{[1
]}

Guo, Zhiyong ^{[2
]}

Chen, Xiaofei ^{[1
]}

机构：

[1] Naval Med Univ, Sch Pharm, 325 Guohe Rd, Shanghai 200433, Peoples R China

[2] Naval Med Univ, Dept Nephrol, Shanghai Changhai Hosp, 168 Changhai Rd, Shanghai 200433, Peoples R China

[3] Chinese Peoples Liberat Army Gen Hosp, Dept Nephrol, Med Ctr 2, 28 Fuxing Rd, Beijing 100853, Peoples R China

[4] Shanghai Jiao Tong Univ, Dept Pharm, Shanghai Peoples Hosp 9, 280 Mohe Rd, Shanghai 201999, Peoples R China

[5] Naval Med Univ, Dept Pharm, Shanghai Eastern Hepatobiliary Surg Hosp, 225 Changhai Rd, Shanghai 200438, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2021年 / 195卷

基金：

中国国家自然科学基金;

关键词：

Comprehensive two-dimensional cell membrane chromatography; Comparative analysis system; Crystal-induced kidney injury; Active ingredients; Pyrrosia calvata (Bak.) Ching; FLIGHT MASS-SPECTROMETRY; EPITHELIAL-CELLS; DRUG DISCOVERY; EXPRESSION; OSTEOPONTIN; COMPONENTS; HYALURONAN; RECEPTORS; MEDICINE;

D O I：

10.1016/j.jpba.2020.113825

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Crystal-induced kidney injury (CIKI) is the fundamental pathological change during nephrolithiasis, although the molecular mechanism is still unclear. Pyrrosia calvata (Bak.) Ching has been used in folk medicine to treat urolithiasis for years. To clarify the pharmacodynamic substances and the mechanism of its antiurolithiasis effects, in this study, a novel, stop-flow, comprehensive, two-dimensional (2D) HK-2 and HK-2/CIKI cell membrane chromatography (CMC) comparative analysis system was developed to screen for the potential active ingredients from Pyrrosia calvata (Bak.) Ching against CIKI. The comprehensive 2D CMC comparative analysis system showed satisfactory selectivity, and eight ingredients were screened and identified by this system. Among them, mangiferin exhibited higher affinity for the HK-2/CIKI CMC column than the HK-2 CMC column and was selected for further efficacy verification. Cell proliferation assays showed that mangiferin could protect HK-2 cell viability after stimulation with sodium oxalate (NaOX). Additionally, in a rodent model of CIKI, mangiferin decreased the deposition of calcium oxalate (CaOX) crystals in mouse kidneys, alleviated the pathological damage to kidney tissue, and inhibited the upregulation of OPN, MCP1, and CD44 expression caused by CaOX crystals. The established comprehensive 2D CMC comparative analysis system can be applied to screen active ingredients with disease specificity from traditional Chinese medicine (TCM) and is suitable for other cell models. (C) 2020 Elsevier B.V. All rights reserved.

引用

页数：8