Synthesis of Lewis X epitopes on plant N-glycans

被引：14
|
作者：
Rouwendal, Gerard J. A. [1 ]
Florack, Dion E. A. [1 ]
Hesselink, Thamara [1 ]
Cordewener, Jan H. [1 ]
Helsper, Johannes P. F. G. [1 ]
Bosch, Dirk [1 ,2 ]
机构：
[1] Univ Wageningen & Res Ctr, Plant Res Int BV, NL-6708 PB Wageningen, Netherlands
[2] Univ Utrecht, Dept Chem, NL-3584 CH Utrecht, Netherlands
来源：
CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 12期
关键词：
Tobacco; Transgenic; beta-(1 -> 4)-Galactosyltransferase; alpha-(1 -> 3)-Fucosyltransferase; N-glycan; Lewis X; DC-SIGN; FUC-TIX; CARBOHYDRATE EPITOPES; BETA-GALACTOSIDASES; SCHISTOSOMA-MANSONI; MOLECULAR-CLONING; TRANSGENIC PLANTS; EXPRESSION; ANTIGEN; ALPHA-1,3-FUCOSYL-TRANSFERASE;
D O I：
10.1016/j.carres.2009.05.003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Glycoproteins from tobacco line xFxG1, in which expression of a hybrid beta-(1 -> 4)-galactosyltransferase (GalT) and a hybrid alpha-(1 -> 3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (Le(X)) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid beta-(1 -> 4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant beta-galactosidase activity. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1487 / 1493
页数：7
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