Myristic acid increases Δ6-desaturase activity in cultured rat hepatocytes

被引：51
|
作者：
Jan, S [1 ]
Guillou, H [1 ]
D'Andrea, D [1 ]
Daval, S [1 ]
Bouriel, M [1 ]
Rioux, V [1 ]
Legrand, P [1 ]
机构：
[1] INRA, ENSA, Biochim Lab, F-35042 Rennes, France
来源：
REPRODUCTION NUTRITION DEVELOPMENT | 2004年 / 44卷 / 02期
关键词：
myristic acid; Delta; 6-desaturase; saturated fatty acids; cultured rat hepatocytes;
D O I：
10.1051/rnd:2004020
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In order to study the effects of saturated fatty acids on Delta6-desaturase activity, rat hepatocytes in primary culture were incubated with lauric (C12:0), myristic (C14:0) or palmitic (C16:0) acids. After optimization, the standard in vitro conditions for the measurement of Delta6-desaturase activity were as follows: 60 mumol.L-1 alpha-linolenic acid (C18:3n-3), reaction time of 20 min and protein content of 0.4 mg. Data showed that cell treatment with 0.5 mmol.L-1 myristic acid during 43 h specifically increased Delta6-desaturase activity. This improvement, reproducible for three substrates of Delta6-desaturase, i.e. oleic acid (C18:ln-9), linoleic acid (C18:2n-6) and alpha-linoleic acid (C18:3n3) was dose-dependent in the range 0.1-0.5 mmol.L-1 myristic acid concentration.
引用
收藏
页码：131 / 140
页数：10
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