Nanoporous gold assembly of glucose oxidase for electrochemical biosensing

被引:61
|
作者
Xiao, Xinxin [1 ]
Ulstrup, Jens [2 ]
Li, Hui [1 ]
Wang, Meng'en [1 ]
Zhang, Jingdong [2 ]
Si, Pengchao [1 ]
机构
[1] Shandong Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Liquid Solid Struct Evolut & Proc Mat, Jinan 250061, Peoples R China
[2] Tech Univ Denmark, Dept Chem, DTU Chem, DK-2800 Lyngby, Denmark
基金
中国国家自然科学基金;
关键词
Nanoporous gold; Self-assembled monolayers; Electron transfer; Glucose oxidase; Electrochemical biosensors; CDTE QUANTUM DOTS; ELECTRON-TRANSFER; CYCLIC VOLTAMMETRY; AU; MONOLAYERS; NANOPARTICLES; OXIDATION; BEHAVIOR; LACCASE; SENSORS;
D O I
10.1016/j.electacta.2014.02.146
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Nanoporous gold (NPG) is composed of three-dimensional (3D) bicontinuous nanostructures with large surface area. Nano-channels inside NPG provide an ideal local environment for immobilization of enzyme molecules with expected stabilization of the protein molecules. In this work, glucose oxidase (GOx) has been brought to assemble on NPG via surface chemical reactions to form enzyme modified NPG nanomaterial with promising sensitivity for glucose detection. Cyclic voltammetry and single-potential step chronoamperometry (SPSC) are employed to study the electrochemical behavior of both bare and enzyme-modified NPG. Two redox mediators, p-benzoquinone (BQ) and ferrocenecarboxylic acid (FCA) are used to shuttle electrons between the enzyme redox center inside of GOx and the NPG electrode. Diffusion patterns at the functionalized NPG electrode are found significantly different from those on planar gold electrodes. This is mainly caused by internal 3D single crystal-like structures of NPG. Electrostatically neutral BQ mediator gives much higher voltammetric sensitivity than negatively charged FCA for GOx modified NPG electrodes. This study provides insight into the understanding of the intrinsic properties of NPG materials aiming at evolving enzymatic biosensors with high performance. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:559 / 567
页数:9
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