Sandwich enzyme-linked immunosorbent assay of canine leptin

被引：30
|
作者：
Iwase, M
Kimura, K
Komagome, R
Sasaki, N
Ishioka, K
Honjoh, T
Saito, M [1 ]
机构：
[1] Hokkaido Univ, Grad Sch Vet Med, Dept Biomed Sci, Biochem Lab, Sapporo, Hokkaido 0600818, Japan
[2] Nippon Vet & Anim Sci Univ, Dept Biochem, Tokyo 1800023, Japan
[3] Morinaga Inst Biol Sci, Tsurumi Ku, Yokohama, Kanagawa 2308504, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2000年 / 62卷 / 02期
关键词：
canine; ELISA; leptin;
D O I：
10.1292/jvms.62.207
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Leptin is the ob gene product secreted from adipocytes in mammals, and thereby its plasma level reflects body fat content. To establish an assay method for leptin in the dog, rabbit anti-canine leptin antibody was obtained using canine recombinant leptin as an antigen. This antibody reacted to canine leptin much stronger than mouse, rat and human leptins. Sandwich enzyme-linked immunosorbent assay (ELISA) using this antibody was developed. The serum leptin levels of 13 healthy dogs were in a range from 1.4 to 5.6 ng/ml with the mean +/- SEM of 3.0 +/- 0.3 ng/ml.
引用
收藏
页码：207 / 209
页数：3
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