Dual antifungal properties of cationic antimicrobial peptides polybia-MPI: Membrane integrity disruption and inhibition of biofilm formation

被引：53

作者：

Wang, Kairong ^{[1
]}

Yan, Jiexi ^{[1
]}

Dang, Wen ^{[1
]}

Xie, Junqiu ^{[1
]}

Yan, Bo ^{[1
]}

Yan, Wenjin ^{[1
]}

Sun, Mengyang ^{[1
]}

Zhang, Bangzhi ^{[1
]}

Ma, Mingxia ^{[1
]}

Zhao, Yanyan ^{[1
]}

Jia, Fengjing ^{[1
]}

Zhu, Ranran ^{[1
]}

Chen, Wei ^{[1
]}

Wang, Rui ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Basic Med Sci, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China

来源：

PEPTIDES | 2014年 / 56卷

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Antifungal activity; Antimicrobial peptide; Polybia-MPI Biofilm formation; CANDIDA-ALBICANS; PAULISTA; VENOM; RESISTANCE; CELLS; MODE;

D O I：

10.1016/j.peptides.2014.03.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

With the increasing emergence of resistant fungi, the discovery and development of novel antifungal therapeutics were urgently needed. Compared with conventional antibiotics, the limited propensity of AMPs to induce resistance in pathogens has attracted great interest. In the present study, the antifungal activity and its mechanism-of-action of polybia-MPI, a cationic peptide from the venom of Social wasp Poly bia Paulista was investigated. We demonstrated that polybia-MPI could potently inhibit the growth of Candida albi cans (C. albicans) and Candida glabrata (C. glabrata). The 50% inhibitory concentrations (IC50) of Polybia-MPI against cancer cells were much higher than the MICs against the tested C. albi cans and C. glabrata cells, indicating that polybia-MPI had high selectivity between the fungal and mammalian cells. Our results also indicated that membrane disturbance mechanism was involved in the antifungal activity. Furthermore, polybia-MPI could inhibit the bio film forming of C. glabrata, which was frequently associated with clinically significant biofilm. These results suggest that polybia-MPI has great advantages in the development of antifungal agents. 2014 Elsevier Inc. All rights reserved.

引用

页码：22 / 29

页数：8

共 1 条

[1] Membrane active antimicrobial activity and molecular dynamics study of a novel cationic antimicrobial peptide polybia-MPI, from the venom of Polybia paulista
Wang, Kairong
Yan, Jiexi
Dang, Wen
Liu, Xin
Chen, Ru
Zhang, Jindao
Zhang, Bangzhi
Zhang, Wei
Kai, Ming
Yan, Wenjin
Yang, Zhibin
Xie, Junqiu
Wang, Rui
PEPTIDES, 2013, 39 : 80 - 88

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