Dyeing performance and antibacterial properties of four novels synthesized reactive dyes (D1-4) were studied in this paper. The dyeing process was achieved under a variety of conditions, embracing concentrations of the dye (1-9%), soda ash (0-25 g L-1), Glauber salt (0-100 g L-1), dyeing temperature (40-90 degrees C), dyeing time (30-90 min), and material to dye liquor ratio (1:05-1:80). The maximum color strength (expressed as K/S) values of the dyed cotton fabrics obtained at 3% (based on fabric weight, wof) dye concentration, 20 g L-1 sodium carbonate, 100 g L-1 Glauber's salt, temperature 80 degrees C (for D-1, D-2 & D-4), and 60 degrees C (for D-3), fixation time 60 min, and material-to-liquor ratio (M/L) 1:10. The fastness properties towards washing, perspiration, rubbing, and light were evaluated. All dyed fabrics showed antibacterial potency against both gram positive (S. aureus) and gram negative (E. coli) bacteria.