Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus

被引：6
|
作者：
Almasi, Mohammad Amin [1 ]
Almasi, Galavizh [1 ]
机构：
[1] Islamic Azad Univ, North Tehran Branch, Young Researchers & Elites Club, Tehran, Iran
来源：
ARCHIVES OF VIROLOGY | 2017年 / 162卷 / 02期
关键词：
Beet necrotic yellow vein virus (BNYVV); DAS-ELISA; IC-RT-LAMP; RT-LAMP; Sugar beet; DIFFERENT VISUALIZING SYSTEMS; POTATO-LEAFROLL-VIRUS; 3 DIAGNOSTIC METHODS; RAPID DETECTION; POLYMYXA-BETAE; SUGAR-BEET; RT-PCR; LAMP; INFECTION; PRODUCTS;
D O I：
10.1007/s00705-016-3116-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.
引用
收藏
页码：495 / 500
页数：6
相关论文
共 50 条
  • [1] Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus
    Mohammad Amin Almasi
    Galavizh Almasi
    [J]. Archives of Virology, 2017, 162 : 495 - 500
  • [2] Development of reverse transcription loop-mediated isothermal amplification assay for rapid detection of an emerging potyvirus: Tomato necrotic stunt virus
    Li, Rugang
    Ling, Kai-Shu
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2014, 200 : 35 - 40
  • [3] A reverse transcription loop-mediated isothermal amplification assay for the detection of strawberry mottle virus
    Ren, Junda
    Wang, Jia
    Zhu, Yadong
    Han, Chenggui
    Shang, Qiaoxia
    [J]. JOURNAL OF PHYTOPATHOLOGY, 2021, 169 (05) : 295 - 302
  • [4] Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid detection of Pepper mottle virus
    Luo, Xiangwen
    Zhang, Deyong
    Zheng, Limin
    Peng, Jing
    Li, Fan
    Zhang, Songbai
    Liu, Yong
    [J]. CANADIAN JOURNAL OF PLANT PATHOLOGY, 2016, 38 (04) : 506 - 510
  • [5] Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay
    Qiao, Ning
    Dai, Huijie
    Liu, Jie
    Zhu, Xiaoping
    Li, Jintang
    Zhang, Dezhen
    Liu, Yongguang
    [J]. PLOS ONE, 2020, 15 (03):
  • [6] Development of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of strawberry crinkle virus
    Ren, Junda
    Zhu, Yadong
    Ran, Ce
    Han, Chenggui
    Shang, Qiaoxia
    [J]. JOURNAL OF PHYTOPATHOLOGY, 2021, 169 (11-12) : 733 - 739
  • [7] Development of a colorimetric reverse transcription loop-mediated isothermal amplification assay for the detection of Mirafiori lettuce big-vein virus
    Almasi, Mohammad Amin
    [J]. ARCHIVES OF VIROLOGY, 2017, 162 (09) : 2775 - 2780
  • [8] Development of a colorimetric reverse transcription loop-mediated isothermal amplification assay for the detection of Mirafiori lettuce big-vein virus
    Mohammad Amin Almasi
    [J]. Archives of Virology, 2017, 162 : 2775 - 2780
  • [9] Development of a reverse transcription loop-mediated isothermal amplification assay for detection of Porcine teschovirus
    Wang, Bin
    Wang, Yongqiang
    Tian, Zhi-Jun
    An, Tong-Qing
    Peng, Jin-Mei
    Tong, Guang-Zhi
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2011, 23 (03) : 516 - 518
  • [10] Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
    Zhang, Zong-Ying
    Liu, Xiao-Jun
    Li, Da-Wei
    Yu, Jia-Lin
    Han, Cheng-Gui
    [J]. VIROLOGY JOURNAL, 2011, 8
12345