Three dimensional (3D) analysis of the morphological changes induced by 50 Hz magnetic field exposure on human lymphoblastoid cells (Raji)

被引：0
|
作者：
Lisi, A
Pozzi, D
Pasquali, E
Rieti, S
Girasole, M
Cricenti, A
Generosi, R
Serafino, AL
Congiu-Castellano, A
Ravagnan, G
Giuliani, L
Grimaldi, S
机构：
[1] CNR, Area Ric, Ist Med Sperimentale, I-00133 Rome, Italy
[2] Univ La Sapienza, Dipartimento Med Sperimentale & Pathol, Rome, Italy
[3] CNR, Ist Psicol, Rome, Italy
[4] CNR, Ist Struttura Mat, Rome, Italy
[5] Polo Biol Integrat, Rome, Italy
[6] Univ La Sapienza, Dipartimento Fis, Rome, Italy
[7] ISPESL, DIDIA, Rome, Italy
来源：
BIOELECTROMAGNETICS | 2000年 / 21卷 / 01期
关键词：
microvilli; ELF; lymphocytes; atomic force microscopy; plasma membrane morphology;
D O I：
10.1002/(SICI)1521-186X(200001)21:1<46::AID-BEM7>3.0.CO;2-Z
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Human Raji B lymphoid cells after exposure for 64 h to a 1 mT (rms) 50 Hz sinusoidal magnetic field showed a reorganization of membrane and cytoskeletal components. Atomic force microscopy in air revealed several modifications in 80% of the exposed cells, such as loss of microvilli-like structures followed by progressive appearance of membrane introflections. This change in plasma membrane morphology was also accompanied by a different actin distribution, as detected by phalloidin fluorescence. These observations support our previous hypothesis that electric and magnetic fields may modify the plasma membrane structure. (C) 2000 Wiley-Liss,Inc.
引用
收藏
页码：46 / 51
页数：6
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