Cohnella lubricantis sp nov., isolated from a coolant lubricant solution

A Gram-stain-positive, aerobic, non-endospore-forming organism, isolated from a coolant lubricant solution was studied for its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain KSS-154-50(T) was grouped into the genus Cohnella, most closely related to Cohnella formosensis CC-ALFALFA-35(T) (97.3% 16S rRNA gene sequence similarity), Cohnella rhizosphaerae CSE-5610(T) (97.1 %) and Cohnella nanjingensis D45(T) (97.0 %); the 16S rRNA gene sequence similarity to other species of the genus Cohnella was < 97.0 %. The fatty acid profile from whole cell hydrolysates was very similar to those reported for other species of the genus Cohnella and supported the allocation to the genus Cohnella. In the fatty acid profiles, iso- and anteiso-branched fatty acids were found as major compounds. The quinone system consisted predominantly of menaquinone MK-7. The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major polyamine is spermidine. The results of physiological and biochemical characterization allowed in addition a phenotypic differentiation of strain KSS-154-50(T) from the three most closely related species. Hence, strain KSS-154-50(T) represents a novel species of the genus Cohnella, for which the name Cohnella lubricantis sp. nov. is proposed. The type strain is KSS154- 50(T) (=LMG 29763(T)=CCM 8707(T)).