Amplification of oak DNA from ancient and modern wood

被引：75
|
作者：
Dumolin-Lapègue, S
Pemonge, MH
Gielly, L
Taberlet, P
Petit, RJ
机构：
[1] INRA, Lab Genet & Ameliorat Arbres Forestiers, F-33611 Gazinet, France
[2] Univ Grenoble 1, CNRS UMR 5553, Lab Biol Populat Altitude, F-38041 Grenoble 9, France
来源：
MOLECULAR ECOLOGY | 1999年 / 8卷 / 12期
关键词：
ancient DNA; chloroplast DNA; DNA isolation; nested PCR; Quercus;
D O I：
10.1046/j.1365-294x.1999.00788.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A polymorphic noncoding region of chloroplast DNA (cpDNA) was successfully amplified by the polymerase chain reaction (PCR) from various oak wood samples, including recent and more ancient (about 600-years-old) samples from different oak species. Adaptation of DNA isolation and amplification protocols was necessary to obtain this result. Polymorphisms useful to distinguish species or geographical origin of these samples could be scored through sequencing. These polymorphisms include one substitution and two microsatellite- type polymorphisms, due to a variable number of A/T repeats, identical results were obtained independently in two separate laboratories.
引用
收藏
页码：2137 / 2140
页数：4
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