Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) SD1 gene

被引:0
|
作者
Su Yuan [1 ]
Xu Shengguang [2 ]
Liu Yonggang [3 ]
机构
[1] Dept Life Sci & Technol, Kunming 650214, Peoples R China
[2] Kunming Univ, Coll Agron, Kunming 650214, Peoples R China
[3] Yunnan Agr Univ, Key Lab Anim Nutr & Feed Yunnan Prov, Kunming 650214, Peoples R China
来源
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2014年 / 9卷 / 11期
关键词
Tobacco; SD1; Tissue expression; STEROL C-22 DESATURASE; STIGMASTEROL; ARABIDOPSIS; TOMATO;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The complete coding sequence of one tobacco (nicotiana tabacum) gene- sterol 22-desaturase (SD1), was amplified based on some tobacco ESTs. The complete coding sequence of tobacco SD1 gene was 1,521bp which encodes a protein of 506 amino acids. Sequence analysis revealed that the SD1 of tobacco shares high homology with the SD1 of lycopersicon esculentum (86%). The prediction of transmembrane helices showed that tobacco SD1 might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco SD1 gene was differentially expressed in detected tobacco tissues including leaf, stem, root and flower.
引用
收藏
页码:47 / 51
页数:5
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