Co-localization of glucokinase with actin filaments

A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose, When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase mas also co-localized with actin filaments in the cytoplasm of these transfected cells, Although colocalization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic beta-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus, These findings suggest that subcellular localization of glucokinase, including colocalization with actin filaments, may have an important physiological role in metabolic regulation. (C) 1997 Federation of European Biochemical Societies.