The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats

The diagnosis of tuberculosis (TB) in goats is based mainly on the single and comparative intradermal tuberculin (SIT and CIT) tests and, exceptionally, on the mterferon-gamma (IFN-gamma assay, however they are not perfect in terms of sensitivity and specificity Nevertheless, various serological assays that provide a potential cost effective approach for the control of TB are also available or under development, and a variety of results have been reported regarding the ability of these tests to detect infected animals, particularly m the early stages of in fection In the present study, SIT/CIT and IFN-y tests and three different serological assays were evaluated during two consecutive herd testing events in a recently infected caprine herd (n = 447) with a high prevalence of infection in order to evaluate their performance and provide field data with which to improve the TB control programs in this species. The proportion of infected animals that tested positive among all the infected goats (T + /I+ value) in the last herd testing event ranged from 26 2% (IC95%; 19.3-34.5) to 85.7% (IC95%, 78.5-90.7) using cell-based diagnostic tests The SIT/SCIT tests detected more infected goats than the IFN-gamma test, regardless of the interpretation criteria The T+/I+ value of serology was 83.2 (IC95%, 75 2-89), although it increased significantly (P < 0.05) when using samples collected 15 days after the intradermal test (100%, IC95%, 97-100). In general, a parallel interpretation of intradermal tests with serology maximized the detection of infected goats. These results demonstrate that serological tests are valuable diagnostic tools to maximize the detection of TB infected goats, even in recent outbreaks, accelerating the eradication process.