Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance

被引：7
|
作者：
Galambos, A. [1 ]
Zok, A. [2 ]
Kuczmog, A. [1 ]
Olah, R. [2 ]
Putnoky, P. [1 ]
Ream, W. [3 ]
Szegedi, E. [4 ]
机构：
[1] Univ Pecs, Fac Sci, Dept Genet & Mol Biol, H-7624 Pecs, Hungary
[2] Corvinus Univ Budapest, Fac Hort Sci, Dept Genet & Plant Breeding, H-1118 Budapest, Hungary
[3] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA
[4] Corvinus Univ Budapest, Inst Viticulture & Enol, H-6000 Kecskemet Miklostelep, Hungary
来源：
PLANT CELL REPORTS | 2013年 / 32卷 / 11期
关键词：
Crown gall; Transgenic grapevine; Agrobacterium tumefaciens; Agrobacterium vitis; Vitis berlandieri x V. rupestris cv. 'Richter 110'; GENETIC-TRANSFORMATION; TUMEFACIENS ONCOGENES; POWDERY MILDEW; VITIS-VINIFERA; T-DNA; DISEASE; PLANTS; REGENERATION; FINGERPRINTS; ROOTSTOCKS;
D O I：
10.1007/s00299-013-1488-0
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Grapevine rootstock transformed with an Agrobacterium oncogene-silencing transgene was resistant to certain Agrobacterium strains but sensitive to others. Thus, genetic diversity of Agrobacterium oncogenes may limit engineering crown gall resistance. Crown gall disease of grapevine induced by Agrobacterium vitis or Agrobacterium tumefaciens causes serious economic losses in viticulture. To establish crown gall-resistant lines, somatic proembryos of Vitis berlandieri x V. rupestris cv. 'Richter 110' rootstock were transformed with an oncogene-silencing transgene based on iaaM and ipt oncogene sequences from octopine-type, tumor-inducing (Ti) plasmid pTiA6. Twenty-one transgenic lines were selected, and their transgenic nature was confirmed by polymerase chain reaction (PCR). These lines were inoculated with two A. tumefaciens and three A. vitis strains. Eight lines showed resistance to octopine-type A. tumefaciens A348. Resistance correlated with the expression of the silencing genes. However, oncogene silencing was mostly sequence specific because these lines did not abolish tumorigenesis by A. vitis strains or nopaline-type A. tumefaciens C58.
引用
收藏
页码：1751 / 1757
页数：7
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