Generation of two human iPSC lines (UUIGPi013-A and UUIPGi014-A) from cases with Down syndrome and full trisomy for chromosome 21 (T21)

被引：2
|
作者：
Schuster, Jens
Hoeber, Jan
Sobol, Maria
Fatima, Ambrin
Anneren, Goran
Dahl, Niklas
机构：
[1] Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden
[2] Uppsala Univ, Sci Life Lab, Uppsala, Sweden
来源：
STEM CELL RESEARCH | 2020年 / 49卷
基金：
瑞典研究理事会;
关键词：
D O I：
10.1016/j.scr.2020.102081
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Down syndrome (DS) is caused by trisomy for chromosome 21 (T21). We generated two induced pluripotent stem cell (iPSC) lines from skin fibroblasts of two males with DS using Sendai virus delivery of OCT4, SOX2, KLF4, and c-MYC. Characterization of the two iPSC lines, UUIGPi013-A and UUIPGi014-A, showed that they are genetically stable with a 47,XY,+21 karyotype. Both lines displayed expression of pluripotency markers and trilineage differentiation capacity. These two iPSC lines provide a useful resource for DS modeling and pharmacological interventions.
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页数：4
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