Evaluation of a blocking ELISA for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus

被引：39

作者：

Sorensen, KJ

Botner, A

Madsen, ES

Strandbygaard, B

Nielsen, J

机构：

[1] Danish Vet. Inst. for Virus Research, Lindholm

来源：

VETERINARY MICROBIOLOGY | 1997年 / 56卷 / 1-2期

关键词：

porcine reproductive and respiratory syndrome virus; PRRS diagnosis virus; antibodies; ELISA;

D O I：

10.1016/S0378-1135(96)01345-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection.

引用

页码：1 / 8

页数：8

共 50 条

[1] Blocking ELISA's for the distinction between antibodies against European and American strains of porcine reproductive and respiratory syndrome virus
Sorensen, KJ
Strandbygaard, B
Botner, A
Madsen, ES
Nielsen, J
Have, P
[J]. VETERINARY MICROBIOLOGY, 1998, 60 (2-4) : 169 - 177
[2] AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION OF ANTIBODIES TO THE PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUS
ALBINA, E
LEFORBAN, Y
BARON, T
DURAN, JP
VANNIER, P
[J]. ANNALES DE RECHERCHES VETERINAIRES, 1992, 23 (02): : 167 - 176
[3] Specificity assessment of an Elisa assay for detection of antibodies against porcine reproductive and respiratory syndrome virus (IDEXX HERDCHEK*PRRS 2XR)
Auvigne, Vincent
Pinsard, Jean-Louis
Gourgues, Herve
[J]. EPIDEMIOLOGIE ET SANTE ANIMALE, NO 55, 2009, (55): : 153 - +
[4] USING THE INDIRECT IMMUNOFLUORESCENCE ASSAY (IIFA) FOR THE DETECTION OF ANTIBODIES AGAINST VIRUS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS)
FICHTNER, D
BERGMANN, S
SCHIRRMEIER, H
[J]. MONATSHEFTE FUR VETERINARMEDIZIN, 1994, 49 (05): : 223 - 227
[5] Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs
Petrini, Stefano
Ramadori, Giorgio
Villa, Riccardo
Borghetti, Paolo
de Angelis, Elena
Cantoni, Anna Maria
Corradi, Attilio
Amici, Augusto
Ferrari, Maura
[J]. VACCINES, 2013, 1 (04):
[6] Gross and histopathological evaluation of reproductive system in sows vaccinated against Porcine Reproductive and Respiratory Syndrome (PRRS)
Papatsiros, V. G.
Alexopoulos, C.
Papaioannou, N.
Kyriakis, S. C.
[J]. REPRODUCTION IN DOMESTIC ANIMALS, 2008, 43 : 119 - 119
[7] Porcine Reproductive and Respiratory Syndrome (PRRS) virus - A significant disease of pigs
Hall, W
[J]. AUSTRALIAN VETERINARY JOURNAL, 2005, 83 (05) : 260 - 261
[8] Detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus in swine sera by enzyme-linked immunosorbent assay
Takikawa, N
Kobayashi, S
Ide, S
Yamane, Y
Tanaka, Y
Yamagishi, H
[J]. JOURNAL OF VETERINARY MEDICAL SCIENCE, 1996, 58 (04): : 355 - 357
[9] Porcine reproductive and respiratory syndrome (PRRS). Review
Maricruz Lopez-Heydeck, Sandra
Alejandro Alonso-Morales, Rogelio
Mendieta-Zeron, Hugo
Carlos Vazquez-Chagoyan, Juan
[J]. REVISTA MEXICANA DE CIENCIAS PECUARIAS, 2015, 6 (01) : 69 - 89
[10] Development and application of a blocking enzyme-linked immunosorbent assay (ELISA) to differentiate antibodies against live and inactivated porcine reproductive and respiratory syndrome virus
Cong, Yanlong
Huang, Zhiqiang
Sun, Yixue
Ran, Wei
Zhu, Lisai
Yang, Guilian
Ding, Xuemei
Yang, Zhanqing
Huang, Xiao
Wang, Chunfeng
Ding, Zhuang
[J]. VIROLOGY, 2013, 444 (1-2) : 310 - 316

← 1 2 3 4 5 →