Cell immobilization on polymer by air atmospheric pressure plasma jet treatment

被引：10

作者：

Lee, Jung-Hwan ^{[1
,2
]}

Kwon, Jae-Sung ^{[1
,2
]}

Om, Ji-yeon ^{[1
]}

Kim, Yong-Hee ^{[3
]}

Choi, Eun-Ha ^{[3
]}

Kim, Kwang-Mahn ^{[1
]}

Kim, Kyoung-Nam ^{[1
,2
]}

机构：

[1] Dept & Res Inst Dent Biomat & Bioengn, Seoul 120752, South Korea

[2] Yonsei Univ, Coll Dent, PLUS Project BK21, Seoul 120752, South Korea

[3] Kwangwoon Univ, Plasma Biosci Res Ctr, Seoul 139701, South Korea

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2014年 / 53卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

ETCHED TITANIUM SURFACE; MODIFIED POLYSTYRENE; IN-VITRO; TOF-SIMS; ADHESION; OSTEOBLAST; XPS; BIOCOMPATIBILITY; CYTOSKELETON; FIBRONECTIN;

D O I：

10.7567/JJAP.53.086202

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The study of cell immobilization on delicate polymer by an air atmospheric pressure plasma jet (AAPPJ) is required for its medical application. The aim of this study was to evaluate whether AAPPJ treatment induce cell immobilization effect on delicate polymers without significant change of surface roughness by AAPPJ treatment. After surface roughness, dynamic contact angle, and chemical characteristics were investigated, the immobilization effect was evaluated with the mouse fibroblast L929 cell line. Surface roughness change was not observed (P > 0.05) in either delicate dental wax or polystyrene plate (PSP) as advancing and receding contact angles significantly decreased (P < 0.05), thanks to decreased hydrocarbon and formation of oxygen-related functional groups in treated PSP. Adherent L929 cells with elongated morphology were found in treated PSP along with the formation of immobilization markers vinculin and actin cytoskeleton. Increased PTK2 gene expression upregulated these markers on treated PSP. (C) 2014 The Japan Society of Applied Physics

引用

页数：7

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