On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

被引:85
|
作者
Aghaamoo, Mohammad [1 ]
Aghilinejad, Arian [2 ]
Chen, Xiaolin [2 ]
Xu, Jie [3 ]
机构
[1] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA USA
[2] Washington State Univ, Sch Engn & Comp Sci, Vancouver, WA USA
[3] Univ Illinois, Dept Mech & Ind Engn, Chicago, IL 60607 USA
关键词
Circulating tumor cell; Continuous separation; Deterministic lateral displacement; Dielectrophoresis; DIELECTRIC-PROPERTIES; LATERAL DISPLACEMENT; PARTICLE SEPARATION;
D O I
10.1002/elps.201800459
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Detection and analysis of circulating tumor cells (CTCs) have emerged as a promising way to diagnose cancer, study its cellular mechanism, and test or develop potential treatments. However, the rarity of CTCs among peripheral blood cells is a big challenge toward CTC detection. In addition, in cases where there is similar size range between certain types of CTCs (e.g. breast cancer cells) and white blood cells (WBCs), high-resolution techniques are needed. In the present work, we propose a deterministic dielectrophoresis (DEP) method that combines the concept of deterministic lateral displacement (DLD) and insulator-based dielectrophoresis (iDEP) techniques that rely on physical markers such as size and dielectric properties to differentiate different type of cells. The proposed deterministic DEP technology takes advantage of frequency-controlled AC electric field for continuous separation of CTCs from peripheral blood cells. Utilizing numerical modeling, different aspects of coupled DLD-DEP design such as the required applied voltages, velocities, and geometrical parameters of DLD arrays of microposts are investigated. Regarding the inevitable difference and uncertainty ranges for the reported crossover frequencies of cells, a comprehensive analysis is conducted on applied electric field frequency as design's determinant factor. Deterministic DEP design provides continuous sorting of CTCs from WBCs even with similar size and has the future potential for high throughput and efficiency.
引用
收藏
页码:1486 / 1493
页数:8
相关论文
共 50 条
  • [1] Design of a novel integrated microfluidic chip for continuous separation of circulating tumor cells from peripheral blood cells
    Maliha Saleem Bakhshi
    Mohsin Rizwan
    Ghulam Jilany Khan
    Hong Duan
    Kefeng Zhai
    [J]. Scientific Reports, 12
  • [2] Design of a novel integrated microfluidic chip for continuous separation of circulating tumor cells from peripheral blood cells
    Bakhshi, Maliha Saleem
    Rizwan, Mohsin
    Khan, Ghulam Jilany
    Duan, Hong
    Zhai, Kefeng
    [J]. SCIENTIFIC REPORTS, 2022, 12 (01)
  • [3] Investigation of a two-step device implementing magnetophoresis and dielectrophoresis for separation of circulating tumor cells from blood cells
    Shamloo, Amir
    Yazdani, Alireza
    Saghafifar, Fatemeh
    [J]. ENGINEERING IN LIFE SCIENCES, 2020, 20 (07): : 296 - 304
  • [4] Design and numerical study on a microfluidic system for circulating tumor cells separation from whole blood using magnetophoresis and dielectrophoresis techniques
    Thi, Y-Van Tran
    Hoang, Bao-Anh
    Thanh, Hang Tran
    Nguyen, Thu-Hang
    Ngoc, Thao Pham
    Thu, Hang Bui
    Hoang, Nam Nguyen
    Bui, Tung Thanh
    Duc, Trinh Chu
    Quang, Loc Do
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2022, 186
  • [5] A Two-Stage Separation of Circulating Tumor Cells Based on Deterministic Lateral Displacement and Dielectrophoresis Techniques
    Wang, Yanjuan
    Wang, Chengxiao
    Zhang, Yunfei
    Han, Meiqi
    Li, Yangjinyu
    Jin, Shanyu
    He, Haiqi
    [J]. IEEE ACCESS, 2021, 9 : 143847 - 143859
  • [6] CONTINUOUS SEPARATION OF CANCER CELLS FROM BLOOD IN A MICROFLUIDIC CHANNEL USING DIELECTROPHORESIS
    Alazzam, Anas
    Stiharu, Ion
    Khashan, Saud
    [J]. PROCEEDINGS OF THE ASME INTERNATIONAL MECHANICAL ENGINEERING CONGRESS AND EXPOSITION, 2014, VOL 10, 2015,
  • [7] Isolation of Circulating Tumor Cells by Dielectrophoresis
    Gascoyne, Peter R. C.
    Shim, Sangjo
    [J]. CANCERS, 2014, 6 (01) : 545 - 579
  • [8] Cascaded spiral microfluidic device for deterministic and high purity continuous separation of circulating tumor cells
    Kim, Tae Hyun
    Yoon, Hyeun Joong
    Stella, Philip
    Nagrath, Sunitha
    [J]. BIOMICROFLUIDICS, 2014, 8 (06):
  • [9] A Dielectrophoresis Based Microdevice for Separation of Cancer Cells from Blood Cells
    Al Ali, Abdulla
    Waheed, Waqas
    Alhammadi, Falah
    Abu-Nada, Eiyad
    Alazzam, Anas
    [J]. 2019 IEEE INTERNATIONAL CONFERENCE ON SENSORS AND NANOTECHNOLOGY (SN), 2019, : 25 - 28
  • [10] SEPARATION OF LEUKEMIA CELLS FROM BLOOD BY EMPLOYING DIELECTROPHORESIS
    Imasato, Hiroko
    Yamakawa, Takeshi
    Eguchi, Masanori
    [J]. INTELLIGENT AUTOMATION AND SOFT COMPUTING, 2012, 18 (02): : 139 - 152